Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments
Keywords:PCR, PKS, NRPS, Actinomycetes, Secondary metabolite
AbstractIn this study, active secondary metabolite production capacity of actinomycete isolates obtained from Trabzon (Black Sea) sea sediments was investigated by molecular techniques. Totaly 24 actinomycetes were investigated by PCR based on the presence of secondary metabolite biosynthesis genes PKS / NRPS. According to the PCR results, 25 and 58% of actinomycetes obtained from Trabzon sea sediments were found to contain PKS-NRPS and only NRPS gene regions, respectively. When PCR data were evaluated, it was found that the production of the peptide form active secondary metabolite of the isolates by non-ribosomal way was higher than that of the secondary metabolite production by the PKS pathway. In addition, it has been determined that Black Sea marine sediments have high potential for active secondary metabolite production.
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