Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L.
Abstract
In this study, various extracts of Hypericum perforatum L. were investigated in terms of their extraction yields. For this purpose, five solvent systems including 70%EtOH-30% H2O (A), 50%EtOH-50% H2O (B), 30%EtOH-70% H2O (C), 100% EtOH (D), and 100% H2O (E) were used. Ultrasonic-assisted extraction was used for extraction and extraction processes were performed at different temperatures (30°-70oC) and extraction times (30-70 min.). The optimum extraction conditions for H. perforatum was determined based on extraction yield, total phenolic contents, and DPPH radical scavenging activities of the crude extracts, as well. The best ultrasound extraction conditions are achieved in the presence of 70%EtOH-30% H2O solvent system, for 40 min, at 30°C for H. perforatum.
Keywords
St. John’s Wort; Hypericum perforatum L.; solvent; extraction time; extraction temperature; optimum condition
Full Text:
PDFDOI: https://doi.org/10.24925/turjaf.v8i1.226-229.3013
This work is licensed under Creative Commons Attribution 4.0 International License
ISSN: 2148-127X

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