The Effect of Adding Different Amounts of Lachancea thermotolerans together with Saccharomyces cerevisiae on Simultaneous Fermentation in Emir Wine Production

Authors

DOI:

https://doi.org/10.24925/turjaf.v11i2.239-245.5422

Keywords:

Lachancea thermotolerans, Saccharomyces cerevisiae, indigenous yeast, co-inoculation, wine, cv. Emir

Abstract

Our current research aimed to investigate the impacts of the use of indigenous yeast Saccharomyces cerevisiae with different amounts of Lachancea thermotolerans yeasts in mixed culture on the general composition, aroma compounds, sensory analysis and yeast growth of cv. Emir wine. The utilization of L. thermotolerans in mixed cultures reduced the total acidity of wines from 5.40 to 5.19 g/L (as tartaric acid). The acidity of high acid grapes musts obtained from various viticulture areas can be relatively decreased in wine production. In addition, there may be a slight decrease in the amount of ethyl alcohol. On the other hand, increasing the inoculum level of L. thermotolerans led to an increase in the amount of higher alcohols. However, the concentration of esters declined with the higher inoculum levels. According to the sensory evaluation, the most preferred wine was the one obtained with co-inoculation of S. cerevisiae and L. thermotolerans strains at the level of 5×106 and 1×108 cells/mL, respectively. As a result, it can be said that the use of L. thermotolerans yeast in different inoculum levels has a positive effect on wine fermentation.

References

Balikci EK, Tanguler H, Jolly NP, Erten H. 2016. Influence of Lachancea thermotolerans on cv. Emir wine fermentation. Yeast, 33: 313-321. https://doi.org/10.1002/yea.3166

Barillère JM, Bénard P. 1986. Exemples d'interprétation de résultats de dégustation. OENO One, 20: 137-154.

Benito Á, Calderón F, Palomero F, Benito S. 2016. Quality and composition of Airén wines fermented by sequential inoculation of Lachancea thermotolerans and Saccharomyces cerevisiae. Food Technology and Biotechnology, 54: 135-144. https://doi.org/10.17113/ftb.54.02.16.4220

Benito S, Hofmann T, Laier M, Lochbühler B, Schüttler A, Ebert K, Rauhut D. 2015. Effect on quality and composition of Riesling wines fermented by sequential inoculation with non-Saccharomyces and Saccharomyces cerevisiae. European Food Research and Technology, 241: 707-717. https://doi.org/10.1007/s00217-015-2497-8

Binati RL, Junior WJL, Luzzini G, Slaghenaufi D, Ugliano M, Torriani S. 2020. Contribution of non-Saccharomyces yeasts to wine volatile and sensory diversity: A study on Lachancea thermotolerans, Metschnikowia spp. and Starmerella bacillaris strains isolated in Italy. International Journal of Food Microbiology, 318, 108470. https://doi.org/10.1016/j. ijfoodmicro.2019.108470

Bokulich NA, Ohta M, Richardson PM, Mills DA. 2013. Monitoring seasonal changes in winery-resident microbiota. PloS one, 8: e66437. https://doi.org/ 10.1371/journal.pone. 0066437

Cabaroglu T. 1995. Nevşehir-Ürgüp yöresinde yetiştirilen Beyaz Emir üzümünün ve bu üzümden elde edilen şarapların aroma maddeleri üzerinde araştırmalar. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora tezi. 164 s. Adana

Cabaroglu T, Canbas A, Baumes R, Bayonove C, Lepoutre JP, Günata Z. 1997. Aroma composition of white wine of Vitis vinifera L. cv. Emir is affected by skin contact. Journal of Food Science, 62: 680-683. https://doi.org/10.1111/j.1365-2621.1997.tb15434.x

Cheraiti N, Guezenec S, Salmon JM. 2005. Redox interactions between Saccharomyces cerevisiae and Saccharomyces uvarum in mixed culture under enological conditions. Applied and Environmental Microbiology, 71: 255-260. https://doi.org/10.1128/AEM.71.1.255-260.2005

Ciani M, Morales P, Comitini F, Tronchoni J, Canonico L, Curiel JA, Gonzalez R. 2016. Non-conventional yeast species for lowering ethanol content of wines. Frontiers in Microbiology, 7: 642. https://doi.org/10.3389/fmicb.2016.00642

Codex, TF. 2008. Turkish food codex communiqué on determining the maximum levels of certain contaminants in foodstuffs. The official gazette, 17(2008), 26879.

Erten H, Campbell I. 2001. The production of low‐alcohol wines by aerobic yeasts. Journal of the Institute of Brewing, 59: 207-215. https://doi.org/10.1002/j.2050-0416.2001.tb00092.x

Erten H, Tanguler H, Cabaroglu T, Canbas A. 2006. The influence of inoculum level on fermentation and flavour compounds of white wines made from cv. Emir. Journal of the Institute of Brewing, 112: 232-236. https://doi.org/10.1002/j.2050-0416.2006.tb00718.x

Etievant PX. 1991. Volatile compounds in food and beverages. In Wine (pp. 483-546). Marcel Dekker New York.

FAO, 2020. Statistical data of grape production. http://www.fao.org/faostat/en/#data

Fleet GH. 1993. Wine microbiology and biotechnology. Harwood Academic Publishers, Chur, Switzerland.

Gobbi M, Comitini F, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M. 2013. Lachancea thermotolerans and Saccharomyces cerevisiae in simultaneous and sequential co-fermentation: a strategy to enhance acidity and improve the overall quality of the wine. Food Microbiology, 33: 271-281. https://doi.org/10.1016/j.fm.2012.10.004

Hirst MB, Richter CL. 2016. Review of aroma formation through metabolic pathways of Saccharomyces cerevisiae in beverage fermentations. American Journal of Enology and Viticulture, 67: 361-370. https://doi.org/10.5344/ajev.2016.15098

Hranilovic A, Albertin W, Capone DL, Gallo A, Grbin PR, Danner L, Jiranek V. 2021. Impact of Lachancea thermotolerans on chemical composition and sensory profiles of Merlot wines. Food Chemistry, 349, 129015. https://doi.org/10.1016/j.foodchem.2021.129015

Hranilovic A, Gambetta JM, Schmidtke L, Boss PK, Grbin PR, Masneuf-Pomarede I, Jiranek V. 2018. Oenological traits of Lachancea thermotolerans show signs of domestication and allopatric differentiation. Scientific Reports, 8: 1-13. https://doi.org/10.1038/s41598-018-33105-7

Jolly NP, Varela C, Pretorius IS. 2014. Not your ordinary yeast: non-Saccharomyces yeasts in wine production uncovered. FEMS Yeast Research, 14: 215-237. https://doi.org/10.1111/ 1567-1364.12111

Jolly NP, Augustyn OPH, Pretorius IS. 2003. The use of Candida pulcherrima in combination with Saccharomyces cerevisiae for the production of Chenin blanc wine. South African Journal of Enology and Viticulture, 24: 63-69. https://doi.org/10.21548/24-2-2641

Kapsopoulou K, Mourtzini A, Anthoulas M, Nerantzis E. 2007. Biological acidification during grape must fermentation using mixed cultures of Kluyveromyces thermotolerans and Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology, 23: 735-739. https://doi.org/10.1007/s 11274-006-9283-5

Marzano M, Fosso B, Manzari C, Grieco F, Intranuovo M, Cozzi G, Santamaria M. 2016. Complexity and dynamics of the winemaking bacterial communities in berries, musts, and wines from Apulian grape cultivars through time and space. PLoS One, 11(6): e0157383. https://doi.org/10.1371/ journal.pone.0157383

Mills DA, Johannsen EA, Cocolin L. 2002. Yeast diversity and persistence in Botrytis-affected wine fermentations. Applied and Environmental Microbiology, 68: 4884-4893. https://doi.org/10.1128/AEM.68.10.4884-4893.2002

Morales ML, Fierro-Risco J, Ríos-Reina R, Ubeda C, Paneque P. 2019. Influence of Saccharomyces cerevisiae and Lachancea thermotolerans co-inoculation on volatile profile in fermentations of a must with high sugar content. Food Chemistry, 276: 427-435. https://doi.org/10.1016/j. foodchem.2018.10.041

Nurgel C, Erten H, Canbas A, Cabaroglu T, Selli S. 2005. Yeast flora during the fermentation of wines made from Vitis vinifera L. cv. Emir and Kalecik Karasi grown in Anatolia. World Journal of Microbiology and Biotechnology, 21: 1187-1194. https://doi.org/10.1007/s11274-006-7002-x

Ough CS, Amerine MA. 1988. Methods for Analysis of Musts and Wines, second edition. Wiley-Interscience, New York, pp. 172–195.

Padilla B, Gil JV, Manzanares P. 2016. Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. Frontiers in Microbiology, 7: 411. https://doi.org/10.3389/ fmicb.2016.00411

Petruzzi L, Capozzi V, Berbegal C, Corbo MR, Bevilacqua A, Spano G, Sinigaglia M. 2017. Microbial resources and enological significance: Opportunities and benefits. Frontiers in microbiology, 8: 995. https://doi.org/10.3389/fmicb.2017. 00995

Pretorius IS. 2000. Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast, 16: 675-729. https://doi.org/10.1002/1097-0061(20000615)16:8 <675::AID-YEA585>3.0.CO;2-B

Querol A, Barrio E, Huerta T, Ramón D. 1992. Molecular monitoring of wine fermentations conducted by active dry yeast strains. Applied and Environmental Microbiology, 58: 2948-2953. https://doi.org/10.1128/aem.58.9.2948-2953.1992

Ribéreau-Gayon P, Glories Y, Maujean A, Dubordie D. 2000. Handbook of Enology, Volume II, The chemistry of wine stabilization and treatments. John Wiley and Sons Ltd., Baffins Lane, Chichester, England, 404 p.

Romano P, Ciani M, Fleet GH. 2019. Yeasts in the Production of Wine (p. 515). New York. NY. USA: Springer. Doi: 10.1007/978-1-4939-9782-4.

Roudil L, Russo P, Berbegal C, Albertin W, Spano G, Capozzi V. 2020. Non-Saccharomyces commercial starter cultures: scientific trends, recent patents and innovation in the wine sector. Recent Patents on Food, Nutrition and Agriculture, 11: 27-39. https://doi.org/10.2174/2212798410666190131103713.

Stewart GG, Russell I. 1998. Brewer’s Yeast: An Introduction to Brewing Science and Technology. Series III, The Institute of Brewing, London, 41-46.

Tempère S, Marchal A, Barbe JC, Bely M, Masneuf-Pomarede I, Marullo P, Albertin W. 2018. The complexity of wine: Clarifying the role of microorganisms. Applied Microbiology and Biotechnology, 102: 3995-4007. https://doi.org/10.1007/s00253-018-8914-8

Unal MU, Sener A. 2016. Correlation between browning degree and composition of important Turkish white wine grape varieties. Turkish Journal of Agriculture and Forestry, 40: 62-67. https://doi.org/10.3906/tar-1412-54

Vaquero C, Loira I, Heras JM, Carrau F, González C, Morata A. 2021. Biocompatibility in ternary fermentations with Lachancea thermotolerans, other non-Saccharomyces and Saccharomyces cerevisiae to control pH and improve the sensory profile of wines from warm areas. Frontiers in Microbiology, 12: 656262. https://doi.org/10.3389/fmicb. 2021.656262

Zea L, Serratosa MP, Mérida J, Moyano L. 2015. Acetaldehyde as a key compound for the authenticity of sherry wines: a study covering 5 decades. Comprehensive Reviews in Food Science and Food Safety, 14: 681-693. https://doi.org/10.1111/1541-4337.12159

Zhang B, Tang C, Yang D, Liu H, Xue J, Duan C, Yan G. 2021. Effects of three indigenous non-Saccharomyces yeasts and their pairwise combinations in co-fermentation with Saccharomyces cerevisiae on volatile compounds of Petit Manseng wines. Food Chemistry, 130807. https://doi.org/10.1016/j.foodchem.2021.130807

Zohre DE, Erten H. 2002. The influence of Kloeckera apiculata and Candida pulcherrima yeasts on wine fermentation. Process Biochemistry, 38: 319-324. https://doi.org/10.1016/S0032-9592(02)00086-9

Downloads

Published

28.02.2023

How to Cite

Tangüler, H., Yetisen, M., Sanyol, A. G., Tuncel, A. E., Turkmaya, T., Bal, V., & Erten, H. (2023). The Effect of Adding Different Amounts of Lachancea thermotolerans together with Saccharomyces cerevisiae on Simultaneous Fermentation in Emir Wine Production. Turkish Journal of Agriculture - Food Science and Technology, 11(2), 239–245. https://doi.org/10.24925/turjaf.v11i2.239-245.5422

Issue

Section

Research Paper