Influences of Rosemary Essential Oil on Some Blood Parameters and Small Instentine Microflora Laying Hens

Abstract

This experiment was designed to investigate the effects of antibiotic, vitamin E and rosemary essential oils in various doses on some blood parameters, beneficial and harmful microorganisms in instentine jejunum microflora of lying hens. The experiment was conducted according to random parcel experiment design. A total of 240 Bovans genotype and 32 weeks aged were equally divided into 6 groups (8 hens in each group with 5 replicates). While the control group was fed with basal diet the treatment groups were supplemented with 500 mg/kg antibiotics; 200 mg/kg vitamin E; 100, 200, and 300 mg/kg REO to basal diet for 90 consecutive days. The result showed that there were no effects of all additions to basal diets on examined blood parameters. Antibiotic and all rosemary essential oils doses to basal diet affected the instentine jejunum bacteria content by decreasing probable coliforms, faecal coliforms, Escherichia coli (E. coli) and Clostridium perfringens (Cl. perfringens) bacteria level significantly. While a 100 mg/kg rosemary essential oils dose was of the same influence with antibiotic (500 mg/kg) on all bacteria species, 200 and 300 mg/kg doses applications were more effective on E. coli compare to antibiotics. 300 mg/kg doses applications dose was more effective on probable faecal coliforms than antibiotics. As conclusion, the results shows the rosemary essential oils has the high antimicrobial effect on some pathogenic bacterias and could be used in laying hen for feed addition.