Determination of Main Plant Sterols in Turkish Bread Wheat (Triticum aestivum L.) by GC-MS

Authors

  • Halil Erdem Soil Science and Plant Nutrition Department, Faculty of Agriculture, Gaziosmanpasa University, 60240 Tokat
  • Yener K. Tosun Plant and Animal Production Department, Vocational School, Bozok University, 66100 Yozgat
  • Mohammed Akkbik Department of Chemistry, Faculty of Arts and Sciences, Bozok University, 66100 Yozgat
  • Orhan Hazer Science and Technology Application and Research Center, Bozok University, 66100 Yozgat

DOI:

https://doi.org/10.24925/turjaf.v5i7.710-719.1035

Keywords:

Turkish bread wheat, β-sitosterol, campesterol, stigmasterol, GC-MS, optimization & validation

Abstract

Plant sterols are belong to triterpenes family of natural products which includes more than 200 different types of plant sterols and more than 4000 other types of triterpenes. The optimization of method, specially the derivatization step as well as the corresponding analytical validation, is the main goal of this study. The optimum temperature, time and reagent volume of derivatization step were obtained at 60°C, 60 minutes and 50 µL, respectively. A rapid and sensitive gas chromatographic–mass spectrometric method was developed and validated for quantitative analysis of the most common plant sterols (β-sitosterol, campesterol and stigmasterol) in 20 Turkish bread wheat cultivars using GC-MS-SIM. Separation of β-cholestanol (I.S), campesterol, stigmasterol and β-sitosterol was achieved on Rxi (5Sil MS) column (60 m×0.25 mm). The limits of detection for β-sitosterol, campesterol and stigmasterol were 0.074, 0.054 and 0.064 mg kg-1, respectively with RSD ≤ 0.66%. The obtained concentrations of campesterol, stigmasterol and β-sitosterol from 20 Turkish bread wheat cultivars ranged from: 15.30 to 76.02, 4.27 to 23.23 and 303.21 to 682.66 mg kg-1, respectively.

Author Biographies

Halil Erdem, Soil Science and Plant Nutrition Department, Faculty of Agriculture, Gaziosmanpasa University, 60240 Tokat

Faculty of Agriculture, Soil Science and Plant Nutrition Department, TR-60240 Tokat, Turkey

Yener K. Tosun, Plant and Animal Production Department, Vocational School, Bozok University, 66100 Yozgat

Vocational School, Plant and Animal Production Department, TR-66100, Yozgat, Turkey

Mohammed Akkbik, Department of Chemistry, Faculty of Arts and Sciences, Bozok University, 66100 Yozgat

Science and Technology Application and Research Center, TR-66100, Yozgat, Turkey

Orhan Hazer, Science and Technology Application and Research Center, Bozok University, 66100 Yozgat

Faculty of Arts and Sciences, Department of Chemistry, TR-66100, Yozgat, Turkey

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Published

21.07.2017

How to Cite

Erdem, H., Tosun, Y. K., Akkbik, M., & Hazer, O. (2017). Determination of Main Plant Sterols in Turkish Bread Wheat (Triticum aestivum L.) by GC-MS. Turkish Journal of Agriculture - Food Science and Technology, 5(7), 710–719. https://doi.org/10.24925/turjaf.v5i7.710-719.1035

Issue

Section

Crop Production