Determination of Total Phenolics, Flavonoids, Carotenoids, β-Carotene and DPPH Free Radical Scavenging Activity of Biscuits Developed with Different Replacement Levels of Pumpkin (Cucurbita maxima) Peel, Flesh and Seeds Powders

Abstract

Pumpkin (Cucurbita maxima Lam.) is a well-known, extensively grown and consumed crop, world-wide. Pumpkins are natural and rich source of potential bioactive compounds. The presence of active phytochemicals makes these fruits a great matrix to be further exploited for therapeutic purposes, beyond biotechnological applications. Peel, flesh and seeds of this fruit are heavily loaded with phenolics, flavonoids and carotenoids, which are the main tributes of this functional and medicinal food. Present study was designed to utilize these parts of pumpkin in the form of powders, at 0, 5, 10 and 15% replacement levels with white flour, to develop biscuits and to obtain methanolic extracts of these biscuits to determine their phytochemical parameters. Among the different treatment biscuits, highest amount of total phenolics (101.79 mg GAE/100 g), flavonoids (60.74 mg CE/ 100 g) and DPPH free radical scavenging activity (38.00 mg AAE/100 g) was found in biscuits with 15% replacement of pumpkin seeds powder, while biscuits with 15% replacement of pumpkin flesh powder exhibited highest amount of total carotenoid contents (6.95 mg/ 100 g) and β carotene (2.86 mg/100 g). Functional biscuits developed from replacement of pumpkin parts powders with wheat flour may be offered to patients facing oxidative stress, degenerative diseases and diabetes. These biscuits can be offered to children for better growth and development. Consumers awareness through proper marketing at commercial level with proper labelling of nutritional facts, may lead to increased demand of this functional and medicinal food rich in bio actives.