The Effects of DGATI-K232A Gene Polymorphisms on Milk Performance Traits in Simmental Cattle
DOI:
https://doi.org/10.24925/turjaf.v12i12.2508-2513.6961Keywords:
DGAT1, K232A, RFLP, Simmental, milk yieldAbstract
Growing world population, scientists aim to achieve high-yielding products by using new techniques and methods in the fields of food, agriculture, and livestock. The aim of this study is to identify the DGAT/K232A gene polymorphism for Enhancing Performance Characteristics in Simmental cattle and to use it in breeding programs. DGAT/K232A gene polymorphism was analyzed by RFLP method in 70 Simmental cattle using CfrI restriction enzyme. The frequency of the K allele was found 0.77, while A allele was 0.23. The distribution of DGAT1-K232A genotype frequencies in the breed was not balanced (p<0.05). No significant relationship was found between DGAT1/K232Agene polymorphisms and milk yield due to the small number of samples.
References
Akbay, C., & Bilgiç, A. (2023). The Effects of Subsidies on the Profitability of Dairy Cattle Farming in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(4), 888-901.
Anggraeni, A. (2019). Genetic polymorphisms of the OLR1 and DGAT1 genes associated with milk components in Holstein Friesian dairy cattle under an intensive management in Central Java. In IOP conference series: earth and environmental science (Vol. 287, No. 1, p. 012001). IOP Publishing.
Anton, I., Kovács, K., Holló, G., Farkas, V., Szabó, F., Egerszegi, I., Rátky, J., Zsolnai, A., & Brüssow, K.-P. (2012). Effect of DGAT1, leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Archives Animal Breeding 55(4):307-314.
Ardicli, S., Soyudal, B., Samli, H., Dincel, D., & Balci, F. (2018). Effect of STAT1, OLR1, CSN1S1, CSN1S2, and DGAT1 genes on milk yield and composition traits of Holstein breed. Revista Brasileira de zootecnia, 47, e20170247.
Babii, A. V., Arkhipova, A. L., Andreichenko, I. N., Brigida, A. V., & Kovalchuk, S. N. (2018). A TaqMan PCR assay for detection of DGAT1 K232A polymorphism in cattle. AIMS Agriculture and Food, 3(3), 306–312. https://doi.org/10.3934/AGRFOOD.2018.3.306
Barton, L., Bureš, D., Kott, T., & Řehák, D. (2016). Associations of polymorphisms in bovine DGAT1, FABP4, FASN, and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls. Meat Science (114) 18-23.
Bhat, S. A., Ahmad, S. M., Ganai, N. A., Khan, S. M., Malik, A., Shah, R. A., ... & Iqbal, Z. (2017). Association of DGAT1, beta-casein and leptin gene polymorphism with milk quality and yield traits in Jersey and its cross with local Kashmiri cattle. Journal of Entomology and Zoology Studies 5(6):557-561.
Bijl, E., de Vries, R., van Valenberg, H., Huppertz, T., & Van Hooijdonk, T. (2014). Factors influencing casein micelle size in milk of individual cows: Genetic variants and glycosylation of κ-casein. International Dairy Journal 34(1):135-141.
Boichard, D., Govignon-Gion, A., Larroque, H., Maroteau, C., Palhiere, I., Tosser-Klopp, G., ... & Brochard, M. (2014). Genetic determinism of milk composition in fatty acids and proteins in ruminants, and selection potential. INRA Productions Animales, 27(4), 283-298.
Cerit, H., Dumen, E., & Sezgin, F. H. (2014). Comparison of DGAT1 K232A polymorphism and its effects on some milk quality parameters in Holstein and native black race cattles. Journal of the Faculty of Veterinary Medicine Kafkas University 20(2):301-305.
Cıtek, J., Brzáková, M., Hanusová, L., Hanuš, O., Večerek, L., Samková, E., ... & Hasoňová, L. (2021). Gene polymorphisms influencing yield, composition and technological properties of milk from Czech Simmental and Holstein cows. Animal Bioscience, 34(1), 2–11.
Conte, G., Mele, M., Chessa, S., Castiglioni, B., Serra, A., Pagnacco, G., & Secchiari, P. I. E. R. (2010). Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle. Journal of Dairy Science, 93(2), 753-763.
Curi, R. A., Chardulo, L. A. L., Arrigoni, M. D. B., Silveira, A. C., & de Oliveira, H. N. (2011). Associations between LEP, DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle. Livestock Science, 135(2-3), 244-250.
Farese Jr, R. V., Cases, S., & Smith, S. J. (2000). Triglyceride synthesis: insights from the cloning of diacylglycerol acyltransferase. Current opinion in lipidology, 11(3), 229-234.
FC, Y. (1999). Microsoft window based freeware for population genetic analysis. Popgene Ver. 1. 31, University of Alberta, Edomonton, AB, Canada.
Gautier, M., Capitan, A., Fritz, S., Eggen, A., Boichard, D., & Druet, T. (2007). Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal of Dairy Science, 90(6), 2980-2988.
Gothwal, A., Magotra, A., Bangar, Y. C., Malik, B. S., Yadav, A. S., & Garg, A. R. (2023). Candidate K232A mutation of DGAT1 gene associated with production and reproduction traits in Indian Dairy cattle. Animal Biotechnology, 34(7), 2608-2616.
Grisart, B., Coppieters, W., Farnir, F., Karim, L., Ford, C., Berzi, P., ... & Snell, R. (2002). Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research, 12(2), 222-231.
Houaga, I., Muigai, A. W., Ng’ang’a, F. M., Ibeagha-Awemu, E. M., Kyallo, M., Youssao, I. A., & Stomeo, F. (2018). Milk fatty acid variability and association with polymorphisms in SCD1 and DGAT1 genes in White Fulani and Borgou cattle breeds. Molecular Biology Reports 45:1849-1862.
Karolyi, D., Vlatka, Č. Č., Salajpal, K., & Marija, D. (2012). The effect of sex and DGAT1 gene polymorphism on fat deposition traits in Simmental beef cattle. Acta Veterinaria 62(1): 91-100.
Kelava, N., Konjačić, M., & Ivanković, A. (2013). Effect of TG and DGAT1 polymorphisms on beef carcass traits and fatty acid profile. Acta Veterinaria 63(1): 89–99.
Kęsek-Woźniak, M. M., Wojtas, E., & Zielak-Steciwko, A. E. (2020). Impact of SNPs in ACACA, SCD1, and DGAT1 genes on fatty acid profile in bovine milk with regard to lactation phases. Animals 10(6):1–13.
Koopaei, H. K., Abadi, M. R. M., Mahyari, S. A., Tarang, A. R., Potki, P., & Koshkoiyeh, A. E. (2012). Effect of DGAT1 variants on milk composition traits in Iranian Holstein cattle population. Animal Science Papers & Reports 30(3): 231-239.
Leskova, L., Bauer, M., Chrenek, P., Lacková, Z., Soročinová, J., Petrovič, V., Kováč, G., & Lešková, M. L. (2013). Detection of DGAT1 gene polymorphism and its effect on selected biochemical indicators in dairy cows after calving. Acta Veterinaria Brno 82(3):265-269.
Li, X., Ekerljung, M., Lundström, K., & Lundén, A. (2013). Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden. Meat Science 94(2): 153–158.
Lu, J., Boeren, S., van Hooijdonk, T., Vervoort, J., & Hettinga, K. (2015). Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome. Journal of Dairy Science 98(5): 3460–3469.
Mach, N., Blum, Y., Bannink, A., Causeur, D., Houee-Bigot, M., Lagarrigue, S., & Smits, M. A. (2012). Pleiotropic effects of polymorphism of the gene diacylglycerol-O-transferase 1 (DGAT1) in the mammary gland tissue of dairy cows. Journal of Dairy Science 95(9): 4989–5000. https://doi.org/10.3168/jds.2012-5348.
Mao, Y. J., Chen, R. J., Chang, L. L., Chen, Y., Ji, D. J., Wu, X. X., Shi, X. K., Wu, H. T., Zhang, M. R., Yang, Z. P., König, S., & Yang, L. G. (2012). Effects of SCD1- and DGAT1-genes on production traits of Chinese Holstein cows located in the Delta Region of Yangtze River. Livestock Science 145(1–3): 280–286.
Mashhadi, H.M., Nassiri, M.R., Mahmoudi, M., Rastin, M., Kashan, N.E.J., Vaez Torshizi, R., Nooraee, S.E. (2012). Polymorphism and sequencing of DGAT1 gene in Iranian Holstein bulls. Iranian Journal of Applied Animal Science 2(1):63-67.
Mohammed, S.A., A. Siham, Rahamtalla, S. Salah, A. Ahmed, Elhafiz, M. Bakhiet, Dousa, and Khalid, M. (2015). DGAT1 Gene in Dairy Cattle. Review Article. Global Journal of Animal Scientific Research 3(1):191-198.
Naslund, J., Fikse, W. F., Pielberg, G. R., & Lundén, A. (2008). Frequency and effect of the bovine acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal of Dairy Science 91(5):2127-2134.
Özkan Ünal, E., Kepenek, E. Ş., Dinç, H., Özer, F., Sönmez, G., Togan, İ. Z., & Soysal, M. İ. (2015). Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds. Turkish Journal of Zoology 39(5): 734–748.
Palombo, V., Milanesi, M., Sgorlon, S., Capomaccio, S., Mele, M., Nicolazzi, E., Ajmone-Marsan, P., Pilla, F., Stefanon, B., & D’Andrea, M. (2018). Genome-wide association study of milk fatty acid composition in Italian Simmental and Italian Holstein cows using single nucleotide polymorphism arrays. Journal of Dairy Science 101(12):11004–11019.
Patel, R.K., Chauhan, J.B., Soni, K.J., Singh, K.M . (2009). Genotype and allele frequencies of DGAT 1 gene in Indian Holstein Bulls. Current Trends In Biotechnology And Pharmacy 3(4): 385-388.
Schennink, A., W. M. Stoop, M. H. P. W. Visker, J. M. L. Heck, H. Bovenhuis, J. J. van der Poel, H. J. F. van Valenberg, and J. A. M. van Arendonk. (2007) DGAT1 underlies large genetic variation in milk‐fat composition of dairy cows. Animal Genetics 38(5): 467-473.
Scotti, E., Fontanesi, L., Schiavini, F., La Mattina, V., Bagnato, A., & Russo, V. (2010). DGAT1 p. K232A polymorphism in dairy and dual purpose Italian cattle breeds. Italian Journal of Animal Science, 9(1), e16.
Smaragdov, M.G. (2011). Association of the DGAT1 gene polymorphism in bulls with cow milk performance. Russian Journal of Genetics 47(1):110-115.
Vanbergue, E., Peyraud, J. L., Guinard-Flament, J., Charton, C., Barbey, S., Lefebvre, R., Gallard, Y., & Hurtaud, C. (2016). Effects of DGAT1 K232A polymorphism and milking frequency on milk composition and spontaneous lipolysis in dairy cows. Journal of Dairy Science 99(7):5739–5749.
Winter, A., Krämer, W., Werner, F. A., Kollers, S., Kata, S., Durstewitz, G., ... & Fries, R. (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences, 99(14), 9300-9305.
Wu, X. X., Yang, Z. P., Shi, X. K., Li, J. Y., Ji, D. J., Mao, Y. J., ... & Gao, H. J. (2012). Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds. Molecular Biology Reports, 39, 1065-1071.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
