Phenolic content and antioxidant activity of hydromethanolic and aqueous extracts of aerial parts of Phlomis crinita.
DOI:
https://doi.org/10.24925/turjaf.v10i10.2061-2066.5173Keywords:
Phlomis crinita, phytochemicals, antioxidant activity, DPPH free radical scavenging assay, ABTS and β-carotene bleaching assay, metal chelation activity, reducing power.Abstract
Phlomis crinita is a plant species of the family Lamiaceae including more than 100 perennial herbs, shrubs, and sub-shrubs species native to the Mediterranean, Central Asia, and India. This species is commonly a good natural source of various secondary metabolites. Therefore, the present study was conducted to determine phenolic content and antioxidant activity of hydromethanolic (PC ME) and aqueous extracts (PC AQE) of aerial parts of P. crinita. Total polyphenols, flavonoids and tannins were quantified, respectively by the methods of Folin-Ciocalteu reagent, aluminum trichloride (AlCl3) and Bate-Smith method. The in vitro antioxidant activity was assessed using DPPH and ABTS•+ radical scavenging, β-carotene-linoleic acid, reducing power and ferrous ion chelating activity assays. PC ME showed high level of tannins (132,13 ±0.68 µg TAE/mg extract) and total phenolic content (82.71±0.79 µg GAE/mg extract), in addition a marked inhibiting oxidation activity of β-carotene/ linoleic acid (74.10%) was observed. Results showed also a higher iron-chelating activity of PC ME (0.20 mg/mL) compared to PC AQE (0.046 mg/mL). The plant extracts revealed a significant antioxidant activity as evidenced by the DPPH and ABTS radical scavenging activity (IC50 = 0.103 mg/mL for PC ME and 0.144 mg/mL for PC AQE) for DPPH assay and (IC50 = 0.0130 mg/mL for PC ME and 0.0187 mg/mL for PC AQE) , as well as the PC ME exhibits higher reducing power (IC50 =0.288mg/mL) than PC AQE (0.296 mg/mL). As a result, P.crinita is suggested as a promising and effective therapeutic medicinal plant for the treatment of several diseases.References
Al-Qudah, M.A., Obeidat, S.M., Saleh, A.M., El-Oqlah, A.A., Al-Masaeed, E., Al-Jaber, H.I., Abu Orabi, S.T., 2018. Volatile Components Analysis, Total Phenolic, Flavonoid Contents, and Antioxidant Activity of Phlomi s Species Collected from Jordan. Journal of Essential Oil Bearing Plants 21, 583–599.
Amor, I.L.-B., Boubaker, J., Sgaier, M. Ben, Skandrani, I., Bhouri, W., Neffati, A., Kilani, S., Bouhlel, I., Ghedira, K., Chekir-Ghedira, L., 2009. Phytochemistry and biological activities of Phlomis species. Journal of ethnopharmacology 125, 183–202.
Bahorun, T., Gressier, B., Trotin, F., Brunet, C., Dine, T., Luyckx, M., Vasseur, J., Cazin, M., Cazin, J.C., Pinkas, M., 1996. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-forschung 46, 1086–1089.
Barhé, T.A., Tchouya, G.R.F., 2016. Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals. Arabian Journal of Chemistry 9, 1–8.
Bate-Smith EC. (1973). Haemanalysis of tannins, the concept of relative astringency. Phytochemistry. 12, 907-912.
Bencheikh, D., Khennouf, S., Bouaziz, A., Baghiani, A., Dahamna, S., Amira, S., Arrar, L., 2016. Antioxidant and antidiabetic activities of the methanolic extract of Olea europaea L. leaves in streptozotocin induced diabetes in rats. International Journal of Pharmacy and Pharmaceutical Research 8, 1347–1357.
Burits, M., Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy research 14, 323–328.
Chirug, L., Nagar, E.E., Okun, Z., Shpigelman, A., 2021. Effect of flavonoid structure and pH on iron-mediated pectin interaction. Food Hydrocolloids 116, 106654.
Cho, S.Y., Kim, M.K., Mok, H., Choo, H., Chong, Y., 2012. Separation of quercetin‘s biological activity from its oxidative property through bioisosteric replacement of the catecholic hydroxyl groups with fluorine atoms. Journal of agricultural and food chemistry 60, 6499–6506.
Chung, Y.-C., Chen, S.-J., Hsu, C.-K., Chang, C.-T., Chou, S.-T., 2005. Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chemistry 91, 419–424.
Couladis, M., Tanimanidis, A., Tzakou, O., Chinou, I.B., Harvala, C., 2000. Essential oil of Phlomis lanata growing in Greece: chemical composition and antimicrobial activity. Planta Medica 66, 670–672.
Dapkevicius, A., Venskutonis, R., van Beek, T.A., Linssen, J.P.H., 1998. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture 77, 140–146.
Decker, E.A., Welch, B., 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and food Chemistry 38, 674–677.
Del Río, L.A., 2015. ROS and RNS in plant physiology: an overview. Journal of Experimental Botany 66, 2827–2837.
Dellai, A., Mansour, H. Ben, Limem, I., Bouhlel, I., Sghaier, M. Ben, Boubaker, J., Ghedira, K., Chekir-Ghedira, L., 2009. Screening of antimutagenicity via antioxidant activity in different extracts from the flowers of Phlomis crinita Cav. ssp mauritanica munby from the center of Tunisia. Drug and chemical toxicology 32, 283–292.
Fernandez, M.T., Mira, M.L., Florencio, M.H., Jennings, K.R., 2002. Iron and copper chelation by flavonoids: an electrospray mass spectrometry study. Journal of Inorganic Biochemistry 92, 105–111.
Ghedadba, N., Hambaba, L., Ayachi, A., Aberkane, M.C., Bousselsela, H., Oueld-Mokhtar, S.M., 2015. Polyphénols totaux, activités antioxydante et antimicrobienne des extraits des feuilles de Marrubium deserti de Noé. Phytothérapie 13, 118–129.
Holze ,C., Michaudel, C ., Mackowiak,C., Haas ,D., Benda, C., Schnepf, D., Wettmarshausen, J., Braun, M. W. D., Leung , Amarasinghe, G. K., Perocchi, F., Staeheli, P. B., and Pichlmair, A., 2017. Oxeiptosis, a ROS-induced caspase-independent
apoptosis-like cell-death pathway. Journal of nature immunologie https://doi.org/10.1038/s41590-017-0013-y
Ismailoglu, U.B., Saracoglu, I., Harput, U.S., Sahin-Erdemli, I., 2002. Effects of phenylpropanoid and iridoid glycosides on free radical-induced impairment of endothelium-dependent relaxation in rat aortic rings. Journal of ethnopharmacology 79, 193–197.
Jamshidi-Kia, F., Lorigooini, Z., Amini-Khoei, H., 2018. Medicinal plants: Past history and future perspective. Journal of herbmed pharmacology 7.
Kabouche, A., Kabouche, Z., Seguin, E., Tillequin, F., Bruneau, C., 2005. phenylethanoid glycoside and flavonoids from Phlomis crinita (Cav.)(Lamiaceae). Biochemical systematics and ecology.
Kandil, O., Radwan, N.M., Hassan, A.B., Amer, A.M.M., El-Banna, H.A., Amer, W.M.M., 1994. Extracts and fractions of Thymus capitatus exhibit antimicrobial activities. Journal of ethnopharmacology 44, 19–24.
Keser, S., Turkoglu, S., Celik, S., Turkoglu, I., 2012. Determination of Antioxidant Capacities of Phlomis pungens Willd. var. hispida Hub.-Mor. Asian Journal of Chemistry 24, 2780.
KoÅ¡inová, P., Di Meo, F., Anouar, E.H., Duroux, J., Trouillas, P., 2011. H-atom acceptor capacity of free radicals used in antioxidant measurements. International Journal of Quantum Chemistry 111, 1131–1142.
MaleÅ¡ev, D., Kuntić, V., 2007. Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions. Journal of the Serbian chemical society 72, 921–939.
Marrkham, K.P., 1982. Techniques of flavonoids identification Academic Press.
Mathiesen, C., Scheen, A.-C., Lindqvist, C., 2011. Phylogeny and biogeography of the lamioid genus Phlomis (Lamiaceae). Kew Bulletin 66, 83–99.
Merouane, M., Saadib, A., Nouia, A.,2018. Impact of removal of micro and nano sized particles on the phenolic contentand antioxidant activity: Study on aqueous and methanolic leaves extracts of Phlomis crinita Industrial Crops & Products 114, 132–136
Merouane, A., Saadi, A., Noui, A., Bader, A., 2019. Evaluation of phenolic contents and antioxidant properties of the leaves and flowers of Phlomis biloba Desf. International Food Research Journal 26, 167–173.
Papuc, C., Goran, G. V, Predescu, C.N., Nicorescu, V., Stefan, G., 2017. Plant polyphenols as antioxidant and antibacterial agents for shelf-life extension of meat and meat products: Classification, structures, sources, and action mechanisms. Comprehensive Reviews in Food Science and Food Safety 16, 1243–1268.
Pham-Huy, L.A., He, H., Pham-Huy, C., 2008. Free radicals, antioxidants in disease and health. International journal of biomedical science: IJBS 4, 89.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine 26, 1231–1237.
Sánchez-Rangel, J.C., Benavides, J., Heredia, J.B., Cisneros-Zevallos, L., Jacobo-Velázquez, D.A., 2013. The Folin–Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination. Analytical Methods 5, 5990–5999.
Sarikurkcu, C., Uren, M.C., Tepe, B., Cengiz, M., Kocak, M.S., 2015. Phlomis armeniaca: Phenolic compounds, enzyme inhibitory and antioxidant activities. Industrial Crops and Products 78, 95–101.
Sarikurkcu, C., Uren, M.C., Tepe, B., Cengiz, M., Kocak, M.S., 2014. Phenolic content, enzyme inhibitory and antioxidative activity potentials of Phlomis nissolii and P. pungens var. pungens. Industrial Crops and Products 62, 333–340.
Sarkhail, P., Nikan, M., Sarkheil, P., Gohari, A.R., Ajani, Y., Hosseini, R., Hadjiakhoondi, A., Saeidnia, S., 2014. Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships. DARU Journal of Pharmaceutical Sciences 22, 1–9.
Sarkhail, P., Rahmanipour, S., Fadyevatan, S., Mohammadirad, A., Dehghan, G., Amin, G., Shafiee, A., Abdollahi, M., 2007. Antidiabetic effect of Phlomis anisodonta: effects on hepatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Pharmacological Research 56, 261–266.
Sharma, S.K., Singh, L., Singh, S., 2013. A review on medicinal plants having antioxidant potential. Indian Journal of Research in Pharmacy and Biotechnology 1, 404.
Yen, G.-C., Hsieh, C.-L., 1998. Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro. Journal of Agricultural and Food chemistry 46, 3952–3957.
Zhang, Y., Wang, Z., 2009. Phenolic composition and antioxidant activities of two Phlomis species: a correlation study. Comptes Rendus Biologies 332, 816–826.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
