PCR Detection and Molecular Characterization of Coxiella burnetii from Rhipicephalus sanguineus Ticks Collected from Dogs
DOI:
https://doi.org/10.24925/turjaf.v12i7.1174-1177.6560Keywords:
Transposase gene, Coxiella burnetii, Rhipicephalus sanguineus, Van, TurkeyAbstract
Coxiella burnetii, the obligate intracellular bacterium, is the causative agent of Q fever, a zoonotic disease of vertebrates including humans. Common ways of infection are breathing in contaminated barn dust and contact with waste from infected animals. The material of this study consists of 200 ticks which were collected from 70 stray dogs in Van province in east of Turkey between June to September of 2019. The collected ticks were transferred to the parasitology laboratory by taking them into tubes containing 70% alcohol and on which labels were affixed. Ticks were placed in tubes and crushed by freezing with liquid nitrogen. DNA was isolated according to the protocol of the kit manufacturer. To detect the DNA of Coxiella burnetii, a Trans 1, Trans 2 primer pair specific for the IS1111 repetitive transposase gene region was used. Bidirectional sequence analysis of the purified amplicons was performed with the DNA Sequencer. As a result of PCR targeting the IS1111 transposase gene, a positive result for Coxiella burnetii was obtained in 2 (1%) of 200 ticks. Potential risk factors and the importance of ticks in the epidemiology of Q fever in free-roaming dogs in Van province were emphasized by identifying the parasitic tick species and the prevalence of C. burnetii positive ticks in dogs.
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