Investigation of Antioxidant Properties of Spartium junceum L.: Effect of Plant Parts and Storage Conditions
DOI:
https://doi.org/10.24925/turjaf.v13i3.564-568.7251Keywords:
Spartium junceum L., antioxidant activity, SOD and CAT enzymes, oxidative stress, storage conditions, MDA levelsAbstract
Spartium junceum L. is a plant traditionally used for different medicinal purposes. While limited research data explicates its antioxidizing ability, interest in this plant is induced mainly due to its possible role, especially against stress-causing oxidative effects. The objectives of this study were to compare antioxidant activity in flowers and leaves of Spartium junceum L., as well as time under different storage conditions implemented for antioxidative mechanisms. Spartium junceum L. plants were obtained from the Kahramanmaras, Turkey region; subsequently, the flowering and leaf parts of the plant were separated and analyzed. Plant homogenates were prepared, and the activities of SOD and CAT enzymes, as well as MDA levels, were determined using spectrophotometric methods. Enzyme activity upon storage at +4°C, -20°C, and -70° temperature enzyme samples were carried out separately and operated for less than one month in our laboratory. Flowers exhibited higher SOD and CAT activities than leaves. Flowers also showed higher levels of MDA. It may be due to the structural and biochemical differences, where flowers experience extra oxidative stress. The optimal enzyme retention under storage conditions was at -70°C, and a decrease in temperature increased the stability of this biocatalyst. In contrast, MDA levels increased at low temperatures at total capacity. The antioxidant properties of the flower extract had stronger antioxidant potential than those of the leaf part, which also means that chemically active substances show much higher concentrations in this plant section. Storage temperature significantly affects the stability of enzymes, and it was stated that low temperatures mainly maintain antioxidant activity. The results obtained from this study recommend Spartium junceum L. as a valuable antioxidant food resource.
References
Abusamra, Y. A., Scuruchi, M., Habibatni, S., Maammeri, Z., Benayache, S., D'Ascola, A., Avenoso, A., Campo, G. M., & Spina, E. (2015). Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line. Pak J Pharm Sci, 28(4), 1225-1232. https://www.ncbi.nlm.nih.gov/pubmed/26142501
Aune, D. (2019). Plant Foods, Antioxidant Biomarkers, and the Risk of Cardiovascular Disease, Cancer, and Mortality: A Review of the Evidence. Adv Nutr, 10(Suppl_4), S404-S421. https://doi.org/10.1093/advances/nmz042
Beslo, D., Golubic, N., Rastija, V., Agic, D., Karnas, M., Subaric, D., & Lucic, B. (2023). Antioxidant Activity, Metabolism, and Bioavailability of Polyphenols in the Diet of Animals. Antioxidants (Basel), 12(6). https://doi.org/10.3390/antiox12061141
Beutler, E. (1984). Red cell metabolism : a manual of biochemical methods (3rd ed.). Grune & Stratton.
Cerchiara, T., Straface, S. V., Chidichimo, G., Belsito, E. L., Liguori, A., Luppi, B., Bigucci, F., & Zecchi, V. (2012). Spartium junceum aromatic water: chemical composition and antitumor activity. Nat Prod Commun, 7(1), 137-140. https://www.ncbi.nlm.nih.gov/pubmed/22428268
Çelik, C. (2023). Abiyotik ve Biyotik Stres Koşullarında Bitkilerde Görev Alan Antioksidanlar. Kendini Yenileyen Tarım. In Z. Sönmez (Ed.), Kendini Yenileyen Tarım (pp. 3-25). Iksad Publications.
Çelik, C., & Pepe, A. V. (2024). Determination of the Biochemical and Antioxidant Enzyme Activities of Rose Oil (Rosa damascena Mill.) Collected in Different Time Periods. Yuzuncu Yıl University Journal of Agricultural Sciences, 34(3), 452-461. https://doi.org/10.29133/yyutbd.1439906
Duman, R., Doğan, H. H., & Karakış, H. (2019). Antiviral Activity of Spartium junceum Against Herpes Simplex Virus Type 1: An In-Vıtro Study. 10(7), 3274-3282.
Eruygur, N., Ayaz, F., Ayyildiz, H. F., & Bagci, Y. (2022). Antioxidant and enzyme inhibition activities of with HPLC-DAD profiling. Emerging Materials Research, 11(4), 464-471. https://doi.org/10.1680/jemmr.22.00016
Fridovich, I. (1995). Superoxide radical and superoxide dismutases. Annu Rev Biochem, 64, 97-112. https://doi.org/10.1146/annurev.bi.64.070195.000525
Habibatni, S., Miceli, N., Ginestra, G., Maameri, Z., Bisignano, C., Cacciola, F., Utczás, M., Mondello, L., Anwar, S., Benayache, S., Zama, D., Benayache, F., & Taviano, M. F. (2016). Antioxidant and antibacterial activity of extract and phases from stems of Spartium junceum L. growing in Algeria. International Journal of Phytomedicine, 8, 37-46.
Jena, A. B., Samal, R. R., Bhol, N. K., & Duttaroy, A. K. (2023). Cellular Red-Ox system in health and disease: The latest update. Biomed Pharmacother, 162, 114606. https://doi.org/10.1016/j.biopha.2023.114606
Karaman, R., & Türkay, C. (2023). Changes in Germination and Quality Characteristics of Mung Bean Seeds Stored for Different Times. Anadolu Tarım Bilimleri Dergisi, 38(3), 581-896. https://doi.org/10.7161/omuanajas.1338713
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193(1), 265-275.
Menghini, L., Massarelli, P., Bruni, G., & Pagiotti, R. (2006). Anti-inflammatory and analgesic effects of L. flower extracts:: A preliminary study. Journal of Medicinal Food, 9(3), 386-390. https://doi.org/DOI 10.1089/jmf.2006.9.386
Nandi, A., Yan, L. J., Jana, C. K., & Das, N. (2019). Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxid Med Cell Longev, 2019, 9613090. https://doi.org/10.1155/2019/9613090
Nanni, V., Canuti, L., Gismondi, A., & Canini, A. (2018). Hydroalcoholic extract of Spartium junceum L. flowers inhibits growth and melanogenesis in B16-F10 cells by inducing senescence. Phytomedicine, 46, 1-10. https://doi.org/10.1016/j.phymed.2018.06.008
Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358. https://doi.org/https://doi.org/10.1016/0003-2697(79)90738-3
Roy, A., Khan, A., Ahmad, I., Alghamdi, S., Rajab, B. S., Babalghith, A. O., Alshahrani, M. Y., Islam, S., & Islam, M. R. (2022). Flavonoids a Bioactive Compound from Medicinal Plants and Its Therapeutic Applications. Biomed Res Int, 2022, 5445291. https://doi.org/10.1155/2022/5445291
Stajner, D., Popovic, B. M., Kapor, A., Boza, P., & Stajner, M. (2010). Antioxidant and scavenging capacity of Anacamptis pyrimidalis L. -Pyrimidal orchid from Vojvodina. Phytother Res, 24(5), 759-763. https://doi.org/10.1002/ptr.3041
Teresa Cerchiara, Giuseppe Blaiotta, Vittoria S. Straface, Emilia Belsito, Angelo Liguori, Barbara Luppi, Federica Bigucci, & Chidichimo, G. (2013). Biological Activity of Spartium junceum L. (Fabaceae) Aromatic Water. Natural Resources, 4(3).
Yesilada, E., Takaishi, Y., Fujita, T., & Sezik, E. (2000). Anti-ulcerogenic effects of flowers on in vivo test models in rats. Journal of Ethnopharmacology, 70(3), 219-226. https://doi.org/Doi 10.1016/S0378-8741(99)00180-4
Yesilada, E., Tsuchiya, K., Takaishi, Y., & Kawazoe, K. (2000). Isolation and characterization of free radical scavenging flavonoid glycosides from the flowers of by activity-guided fractionation. Journal of Ethnopharmacology, 73(3), 471-478. https://doi.org/Doi 10.1016/S0378-8741(00)00327-5
Younus, H. (2018). Therapeutic potentials of superoxide dismutase. Int J Health Sci (Qassim), 12(3), 88-93. https://www.ncbi.nlm.nih.gov/pubmed/29896077
Zengin, G., Mahomoodally, M. F., Picot-Allain, C. M. N., Cakmak, Y. S., Uysal, S., & Aktumsek, A. (2019). In vitro tyrosinase inhibitory and antioxidant potential of Consolida orientalis, Onosma isauricum and Spartium junceum from Turkey. South African Journal of Botany, 120, 119-123. https://doi.org/10.1016/j.sajb.2018.01.010
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