Evaluation of Antioxidant and Anti-inflammatory Potential of Alpinia officinarum with Different Ionic Solutions
DOI:
https://doi.org/10.24925/turjaf.v13i3.559-563.7361Keywords:
Alpinia officinarum, antioxidant activity, SOD and CAT enzymes, oxidative stress, MDA levels, Ionic SolutionsAbstract
Alpinia officinarum, which attracts attention with its antioxidant and anti-inflammatory properties, is used in traditional medicine, especially to relieve stomach and digestive system disorders. Although many studies have revealed the various pharmacological effects of Alpinia officinarum, the effect of different ionic solvents on its biological activities has yet to be investigated. In this study, the effects of homogenization of Alpinia officinarum roots with potassium chloride (KCl), sodium chloride (NaCl), and phosphate (PBS) buffer solutions on the antioxidant and anti-inflammatory properties of the plant were investigated. Alpinia officinarum plant was collected from the Adana region during the season, and fresh root parts were separated and analyzed. Superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) enzyme activities, and malondialdehyde (MDA) levels of plant homogenates prepared with KCl, NaCl, and PBS were determined by spectrophotometric analysis. The highest MPO and CAT enzyme activities were observed in the KCl solution, while lower levels were observed in NaCl and PBS solutions, respectively. The highest MDA level was observed in the PBS solution. Moreover, SOD enzyme activity showed a decreasing trend in NaCl, KCl, and PBS solutions, respectively. These findings suggest that the biological activity of plant extracts may vary depending on the solvent used. Determination of the conditions under which the antioxidant and anti-inflammatory effects of Alpinia officinarum in different ionic solvents are the highest supports increasing the bioavailability of the plant.
References
Abd Rahman, I. Z., Adam, S. H., Hamid, A. A., Mokhtar, M. H., Mustafar, R., Kashim, M., Febriza, A., & Mansor, N. I. (2024). Potential Neuroprotective Effects of Alpinia officinarum Hance (Galangal): A Review. Nutrients, 16(19). https://doi.org/10.3390/nu16193378
Aghaei, K., Ehsanpour, A. A., & Komatsu, S. (2009). Potato responds to salt stress by increased activity of antioxidant enzymes. J Integr Plant Biol, 51(12), 1095-1103. https://doi.org/10.1111/j.1744-7909.2009.00886.x
Ashtari, A., Niazvand, F., Chamkouri, N., Mohammadi, A., & Karami, A. B. (2023). The ameliorative effects of Alpinia officinarum rhizome hydroalcoholic extract on cisplatin-induced testicular toxicity in rats. JBRA Assist Reprod, 27(1), 41-48. https://doi.org/10.5935/1518-0557.20220017
Beutler, E. (1984). Red cell metabolism : a manual of biochemical methods (3rd ed.). Grune & Stratton.
Çelik, Ö., & Atak, Ç. (2012). The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties content of two Turkish tobacco varieties Turkish Journal of Biology, 36, 339-356.
Dichala, O., Giannakoula, A. E., & Therios, I. (2022). Effect of Salinity on Physiological and Biochemical Parameters of Leaves in Three Pomegranate (Punica Granatum L.) Cultivars. Applied Sciences, 12(17), 8675. https://www.mdpi.com/2076-3417/12/17/8675
F, A. A., Assirey, E. A., El-Meligy, R. M., Awaad, A. S., El-Sawaf, L. A., Allah, M. M., & Alqasoumi, S. I. (2019). Analysis of Alpina officinarum Hance, chemically and biologically. Saudi Pharm J, 27(8), 1107-1112. https://doi.org/10.1016/j.jsps.2019.09.007
Frangie, C., & Daher, J. (2022). Role of myeloperoxidase in inflammation and atherosclerosis (Review). Biomed Rep, 16(6), 53. https://doi.org/10.3892/br.2022.1536
Fridovich, I. (1995). Superoxide radical and superoxide dismutases. Annu Rev Biochem, 64, 97-112. https://doi.org/10.1146/annurev.bi.64.070195.000525
Ghil, S. (2013). Antiproliferative activity of Alpinia officinarum extract in the human breast cancer cell line MCF-7. Mol Med Rep, 7(4), 1288-1292. https://doi.org/10.3892/mmr.2013.1305
Honmore, V. S., Kandhare, A. D., Kadam, P. P., Khedkar, V. M., Sarkar, D., Bodhankar, S. L., Zanwar, A. A., Rojatkar, S. R., & Natu, A. D. (2016). Isolates of Alpinia officinarum Hance as COX-2 inhibitors: Evidence from anti-inflammatory, antioxidant and molecular docking studies. International Immunopharmacology, 33, 8-17. https://doi.org/https://doi.org/10.1016/j.intimp.2016.01.024
Javaid, F., Mehmood, M. H., & Shaukat, B. (2021). Hydroethanolic Extract of A. officinarum Hance Ameliorates Hypertension and Causes Diuresis in Obesogenic Feed-Fed Rat Model. Front Pharmacol, 12, 670433. https://doi.org/10.3389/fphar.2021.670433
Lei, X., Wang, J., Zuo, K., Xia, T., Zhang, J., Xu, X., Liu, Q., & Li, X. (2024). Alpinia officinarum Hance: a comprehensive review of traditional uses, phytochemistry, pharmacokinetic and pharmacology. Front Pharmacol, 15, 1414635. https://doi.org/10.3389/fphar.2024.1414635
Li, C. Y., Cheng, S. E., Wang, S. H., Wu, J. Y., Hsieh, C. W., Tsou, H. K., & Tsai, M. S. (2021). The Anti-inflammatory Effects of the Bioactive Compounds Isolated from Alpinia officinarum Hance Mediated by the Suppression of NF-kappaB and MAPK Signaling. Chin J Physiol, 64(1), 32-42. https://doi.org/10.4103/CJP.CJP_81_20
Lin, K., Deng, T., Qu, H., Ou, H., Huang, Q., Gao, B., Li, X., & Wei, N. (2023). Gastric protective effect of Alpinia officinarum flavonoids: mediating TLR4/NF-kappaB and TRPV1 signalling pathways and gastric mucosal healing. Pharm Biol, 61(1), 50-60. https://doi.org/10.1080/13880209.2022.2152058
Lin, K., Wang, Y., Gong, J., Tan, Y., Deng, T., & Wei, N. (2020). Protective effects of total flavonoids from Alpinia officinarum rhizoma against ethanol-induced gastric ulcer in vivo and in vitro. Pharm Biol, 58(1), 854-862. https://doi.org/10.1080/13880209.2020.1803370
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193(1), 265-275.
Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358. https://doi.org/https://doi.org/10.1016/0003-2697(79)90738-3
Ozkan, G., Kamiloglu, S., Ozdal, T., Boyacioglu, D., & Capanoglu, E. (2016). Potential Use of Turkish Medicinal Plants in the Treatment of Various Diseases. Molecules, 21(3), 257. https://www.mdpi.com/1420-3049/21/3/257
Page, M. J., & Di Cera, E. (2006). Role of Na+ and K+ in enzyme function. Physiol Rev, 86(4), 1049-1092. https://doi.org/10.1152/physrev.00008.2006
Panuccio, M. R., Jacobsen, S. E., Akhtar, S. S., & Muscolo, A. (2014). Effect of saline water on seed germination and early seedling growth of the halophyte quinoa. AoB Plants, 6. https://doi.org/10.1093/aobpla/plu047
Rajendiran, V., Natarajan, V., & Devaraj, S. N. (2018). Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models. Food Science and Human Wellness, 7(4), 273-281. https://doi.org/https://doi.org/10.1016/j.fshw.2018.10.004
Suja, S., & Chinnaswamy, P. (2008). Inhibition of in vitro cytotoxic effect evoked by Alpinia galanga and Alpinia officinarum on PC - 3 cell line. Anc Sci Life, 27(4), 33-40. https://www.ncbi.nlm.nih.gov/pubmed/22557284
Xin, N. (2011). Effect of oils in alpinia officinarum hance on serum NO,SOD,MDA in gastrelcosis mice model. China Journal of Traditional Chinese Medicine and Pharmacy.
Yao, S., Chen, S., Xu, D., & Lan, H. (2010). Plant growth and responses of antioxidants of Chenopodium album to long-term NaCl and KCl stress. Plant Growth Regulation, 60(2), 115-125. https://doi.org/10.1007/s10725-009-9426-4
Zhao, Y.-x., Ruan, W.-j., Xue, W.-l., & Zhao, L. (2019). Alpinia officinarum Hance extract alleviates particulate matter-induced lung injury in mice. Asian Pacific Journal of Tropical Medicine, 12(12), 565-573. https://doi.org/10.4103/1995-7645.272487
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