Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology

Yazarlar

  • Ceren Sunguc Department of Biotechnology and Bioengineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Izmir
  • Oguz Bayraktar Department of Chemical Engineering, Faculty of Engineering, Ege University, 35040 Izmir
  • İpek Erdogan Department of Biotechnology and Bioengineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Izmir
  • Mehmet Emin Uslu Department of Biomedical Equipment Technology, Soma Vocational School, Celal Bayar University, 45500 Manisa

DOI:

https://doi.org/10.24925/turjaf.v5i7.792-799.1195

Anahtar Kelimeler:

Extraction parameters- hyperoside- isoquercetin- phenolic content- prickly shrubby burnet- Sarcopoterium spinosum

Özet

The shrublands are very common in Urla-Çeşme-Karaburun peninsula located in the western point of Turkey. Prickly shrubby burnet (Sarcopoterium spinosum L.) is one of the common weed which has intensive thorns making its consumption for the local domestic animals. However, Sarcopoterium spinosum is a valuable and common medicinal plant in the Mediterranean region. Crude extract of S. spinosum leaves exhibited higher antioxidant activity, as 3143.5± 238.5 µM TEAC (Trolox Equivalent Antioxidant Capacity)/g dry weight (DW), when compared to other medicinal plants found in the literature. The aim of this study was to determine the effect of extraction parameters on the content and biological activity of the extract by response surface methodology (RSM) as well as to identify its major compounds. High Performance Liquid Chromatography (HPLC) was employed to investigate the phenolic content of S. spinosum extract. The composition of the phenolic contents including hyperoside and isoquercetin, the latter being the major component, in S. spinosum extract has been shown for the first time by HPLC. Antimicrobial activity of S. spinosum extract, identified by minimum inhibition concentration (MIC) assay, indicated that the crude extract had antifungal activity against Candida albicans.

Yayınlanmış

2017-07-22

Nasıl Atıf Yapılır

Sunguc, C., Bayraktar, O., Erdogan, İpek, & Uslu, M. E. (2017). Optimization of Extraction Parameters of Phenolic Compounds from Sarcopoterium spinosum Leaves by Response Surface Methodology. Türk Tarım - Gıda Bilim Ve Teknoloji Dergisi, 5(7), 792–799. https://doi.org/10.24925/turjaf.v5i7.792-799.1195

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