The Development of Optimal Cryopreservation Media For Longspine Scraper (Capoeta trutta) Sperm

Erdinç Şahinöz; Zafer Doğu; Faruk Aral

doi:10.24925/turjaf.v6i3.380-386.1766

Yazarlar

Erdinç Şahinöz Department of Fisheries and Aquaculture, Bozova Vocational High School, Harran University, 63850 Şanlıurfa
Zafer Doğu Department of Fisheries and Aquaculture, Bozova Vocational High School, Harran University, 63850 Şanlıurfa
Faruk Aral Department of Reproduction and Artificial Insemination, Bor Vocational High School, Niğde Ömer Halisdemir University, 51700 Niğde

DOI:

https://doi.org/10.24925/turjaf.v6i3.380-386.1766

Anahtar Kelimeler:

Capoeta trutta- longspine scraper- sperm- cryopreservation

Özet

This study is performed to determine some of sperm quality after applying freezing / thawing process. Thus, the aim of this study is to examine different cryprotective agents with additives in terms of their effects at different pH on the cryopreservation process of longspine scraper (Capoeta trutta). The present study, twelve media were prepared by mixing three different cryoprotectants (dimethyl sulfoxide (DMSO, (CH3)2SO); methanol (CH3OH); methyl glycol (MG, CH3O (CH2)2OH)) with an extenders (glucose) at four different pH (7.2, 7.6, 8.0 and 8.4) for longspine scraper semen. Considering the findings from the examination (The motility rate after thawing process and duration of motility obtained in DMSO as 81% and 20 min, in methanol as 73% and 12 min, in methyl glycol as 60% and 15 min.), we can conclude that the DMSO is the best freezing media in order to create new essays in cryopreservation for sperm of Capoeta trutta in the future.