Effect of Different Hormones Concentration on In vitro Regeneration of Apricot Cultivars
DOI:
https://doi.org/10.24925/turjaf.v11i8.1372-1379.6194Anahtar Kelimeler:
Apricot- hasanbey- hacıhaliloğlu- kabaaşı- RegenerationÖzet
The use of modern breeding methods along with appropriate cultivation techniques facilitates the acquisition and multiplication of varieties that possess the desired characteristics. Therefore, efforts towards the in vitro propagation of woody plants are increasing day by day. Today, plants such as Malus, Prunus, Pyrus, Ribes, Rubus, etc., can be successfully propagated in vitro. Apricot stands out as a stubborn species among Prunus types for shoot regeneration and genetic transformation. In this context, this study aims to determine how different plant growth regulators affect shoot regeneration of some native apricot varieties, which hold significant importance in apricot cultivation in our country. In the conducted study, mature cotyledons of Kabaaşı, Hacıhaliloğlu, and Hasanbey apricot varieties were used along with the culture medium consisting of MS. Different doses and ratios of plant growth regulators, including BAP and TDZ, in combination with NAA and GA3, were added to the culture media. At the end of the in vitro study, the callus and primordium formation rate (%), bud and shoot formation rate (%) and number of shoots per explant were recorded. According to the results, the variety with the highest callus formation was Kabaaşı, followed by Hasanbey and Hacıhaliloğlu. In all three varieties, the rate of callus formation decreased in media containing GA3. Regarding the stage of shoot regeneration from callus, the highest shoot formation with an average of 4 shoots per explant was observed in the Kabaaşı variety in the TDZ (1.0 mgL-1) + NAA (0.25 mgL-1) and TDZ (1.0 mgL-1) + NAA (0.50 mgL-1) media. Looking at the other varieties, the highest number of shoots, 1.6 shoots per explant, was obtained from the TDZ (2.0 mgL-1) + NAA (0.25 mgL-1) medium in Hasanbey and Hacıhaliloğlu varieties. As a result of the findings, the Kabaaşı variety showed the best result in terms of the regeneration capacity of apricot varieties. In contrast the best regeneration medium was obtained from the combinations of TDZ and NAA.
Referanslar
Bhagwat B, Dawid Lane W. 2004. In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) ‘Lapins’ and ‘Sweetheart’. Plant Cell Tissue Organ Culture, 78: 173- 181. Doi: 10.1023/B:TICU.0000022552.12449.71
Bhaskaran S, Smith RH. 1990. Regeneration in cereal tissue culture: A Review. Crop Science, 20, 1328-1336. Doi: 10.2135/cropsci1990.0011183X003000060034x
Birsin MA, Önde S, Özgen M. 2001. Callus induction and plant regeneration from mature embryos of oat. Turkish Journal of Biology, 25, 427-434.
Canlı F, Tian L. 2008a. In vitro shoot regeneration from stored mature cotyledons of sweet cherry (Prunus avium L.) cultivars. Scientia Horticulturae, 116: 34-40. Doi: 10.1016/j.scienta.2007.10.023
Canlı F, Tian L. 2008b. Regeneration of adventitious shoots from mature stored cotyledons of Japanese plum (Prunus salicina Lind). Scientia Horticulturae, 120: 64-69. Doi: 10.1016/j.scienta.2008.09.017
Canlı F, Tian L. 2009a. Assessment of regeneration and transient expression factors for agrobacterium-mediated transformation of Prunus Salicina Lind. European Journal of Horticultural Sciences, 74: 66-72.
Canlı FA. 2009b. Effects of standard basal salt mixtures and 1-Phenyl-3-(1,2,3-thiadizazol-5-yl) urea pre-treatments on shoot regeneration from mature cotyledons of sweet cherry in vitro. Advanced Journal of Chemistry, 21: 697-702.
De Bondt A, Eggermont K, Pennickx I, Goderis I, Broekaert WF. 1996. Agrobacterium-mediated transformation of apple (Malus×domestica Borkh.): An assessment of factors affecting regeneration of transgenic plants. Plant Cell Reports, 15: 549–554. Doi: 10.1007/BF00232992
Dinani ET. 2018. Multiplication of Apple ‘Gala’ (Malus domestica L.) by Thidiazuron (Germination, In vitro root-based regeneration, and micrografting). Master Thesis, The University of Guelph, Ontario, Canada.
Erturan H. 2008. In vitro Koşullar Altında Bazı Kayısı (Prunus armeniaca L.) Çeşitlerinden Elde Edilen Olgun Embriyolardan Batkı Rejenerasyonu. Fen Bilimleri Enstitüsü, Atatürk Üniv., Erzurum, Türkiye.
Escalettes V, Dosba F. 1993. In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Science, 90(2):201-209. Doi: 10.1016/0168-9452(93)90241-Q
Hammatt N, Grant NJ. 1998. Shoot regeneration from leaves of Prunus serotina Ehrh. (Black cherry) and P. avium L. (wild cherry). Plant Cell Report, 17: 526-530. Doi: 10.1007/s002990050436
Marcotrigiano M, Gradziel TM. 1997. Genetic mosaics and plant improvement. Plant Breeding Reviews, 15: 43- 84.
Hatipoğlu R. 1999. Bitki Biyoteknolojisi. Ders Kitapları. Yayın No: A-58.
Heslop-Harrison J. 2005. Introduction. P. XIX-XXIV. In: R. E. Litz (ed.). Biotechnology of Fruit and Nut Crops. CABI Publishing, Trowbridge.
Jain SM. 2001. Tissue culture-derived variation in crop improvement. Euphytica, 118: 153-166. Doi: 10.1023/A:1004124519479
Matt A, Jehle JA. 2005. In Vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.). Plant Cell Report, 24: 468- 476. Doi: 10.1007/s00299-005-0964-6
Mendi YY, Çömlekçioğlu N, İpek M, Kocaman E, İzgü T, Tekdal D, Çürük P. 2010. The effect of different hormone concentrations and dark pretreatment on adventitious shoot regeneration in snake melon. Romanian Biotechnological Letters Vol. 15, No. 4, 3592-3595.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15:473- 497.
Leblay C, Chevreau E, Raboin LM. 1990. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.) Plant Cell Tissue and Organ Culture, 25: 99-105. Doi: 10.1007/BF00042180
Litz RE, Gray DJ. 1992. Organogenesis and somatic embryogenesis. P. 3-34. In: F. A. Hammerschlag and R. E. Litz (eds). Biotechnology of Perennial Fruit Crops. CABI, University Press, Cambridge.
Özcan S, Barghchi M, Firek S, Draper J. 1993. Efficient adventitious shoot regeneration and somatic embryogenesis in pea. Plant Cell, Tissue and Organ Culture, 11: 44-47. Doi: 10.1007/BF00029716
Özcan S, Sevimay CS, Yıldız M, Sancak C, Özgen M. 1996. Prolific shoot regeneration from immature embryo explants of sainfoin (Onobrychis viciifolia Scop.). Plant Cell Report, 16: 200-203. Doi: 10.1007/BF01890867
Özçağıran, R, Ünal A, Özeker E, İsfendiyaroğlu M. 2005. Ilıman İklim Meyve Türleri: Sert Çekirdekli Meyveler Cilt-I. Ege Üniversitesi Ziraat fakültesi Yayınları, No:553.
Sancak C. 1999. Koca fiğ (Vicia narbonensis L.)’in olgunlaşmamış embriyo eksplantlarından adventif sürgün rejenerasyonu. Gazi Üniv. Gazi Eğitim Fak. Dergisi, 19, 25-33.
Sarwar M, Skirvin RM. 1997. Effect of thidiazuron and 6-benzylaminopurine on adventitious shoot regeneration from leaves of three strains of ‘McIntosh’ apple (Malus X domestica Borkh.) in vitro. Scientia Horticulturae, 68:95-100. Doi: 10.1016/S0304-4238(96)00971-5
Schuerman PL, Dandekar AM. 1993. Transformation of temperate woody crops: Progress and potentials. Scientia Horticulturae, 55: 101-124. Doi: 10.1016/0304-4238(93)90027-N
Tang H, Ren Z, Reustle G, Krczal G. 2002. Plant regeneration from leaves of sweet and sour cherry cultivars. Scientia Horticulturae, 93:235-244. Doi: 10.1016/S0304-4238(01)00328-4
Taştepe MR. 2011. İn vitro Koşullar Altında Çilekte (FragariaXananasa) Rejenerasyon Üzerine Farklı Uygulamaların Etkisi. Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Atatürk Üniv., Erzurum, Türkiye.
Uranbey S, Çöçü S, Sancak C, Parmaksız İ, Khawar KM, Mirici S, Özcan S. 2003. Adventitious shoot regeneration in cicer milkvetch. Biotechnology & Biotechnological Equipment, 17: 33-37. Doi: 10.1080/13102818.2003.10819192
Wang H, Petri C, Burgos L, Alburquerque N. 2013. Efficient in vitro shoot regeneration from mature apricot (Prunus armeniaca L.) cotyledons. Scientia Horticulturae, 160, 300-305. Doi: 10.1016/j.scienta.2013.06.013
Werbrouck PO, Debergh PC. 1994. Applied aspects of plant regeneration. 6A. micropropagation. In: Dixon RA, Gonzales RA (eds), Plant Cell Culture. A Practical Approach. Second Edition, pp. 127-135, Oxford Pres, UK.
Yıldırım H. 2006. Hacıhaliloğlu Kayısı (Prunus armeniaca L.) Çeşidinin İn Vitro Çoğaltımı. Doktora Tezi, Fen Bilimleri Enstitüsü, Dicle Üniv., Diyarbakır, Türkiye.
İndir
Yayınlanmış
Nasıl Atıf Yapılır
Sayı
Bölüm
Lisans
Bu çalışma Creative Commons Attribution-NonCommercial 4.0 International License ile lisanslanmıştır.
