Bazı Tıbbi ve Aromatik Bitki Ekstraktlarının Antibiyotik Dirençli Escherichia coli Suşları Üzerine Antimikrobiyal Aktivitelerinin Belirlenmesi
DOI:
https://doi.org/10.24925/turjaf.v12i11.1938-1943.7096Anahtar Kelimeler:
Tıbbi ve aromatik bitkiler- Zencefil- Sarımsak- Nane- Antibiyotik direnç- Escherichia coliÖzet
Bu çalışmada zencefil (Zingiber officinale), sarımsak (Allium sativum L.) ve nane (Mentha piperita L.) tıbbi ve aromatik bitkilerinden farklı çözücüler kullanılarak hazırlanan ekstraktların çoklu antibiyotik dirençli Escherichia coli suşları üzerine in vitro antimikrobiyal aktivitesi belirlenmiştir. Zencefil, sarımsak ve nane ekstraktlarının hazırlanmasında çözücü olarak etanol, metanol, aseton ve saf su kullanılmıştır. Çözücülerin ekstraksiyon verimi belirlendikten sonra hazırlanan ekstraktların in vitro antimikrobiyal aktivitesi altı farklı çoklu antibiyotik dirençli E. coli suşu (EC1-6) kullanılarak disk difüzyon yöntemiyle belirlenmiştir. Çözücü olarak su veya metanol kullanılan nane ekstraksiyonundan elde edilen ekstraksiyon verimi, aseton ve etanolden daha yüksek bulunmuştur (P<0,001). Benzer şekilde, zencefil ve sarımsak örneklerinden su kullanılarak alınan ekstraksiyon verimi diğer çözücülerden daha yüksek olmuştur (sırasıyla P=0,013 ve P<0,001). Zencefil etanol ve metanol ekstraktlarının E. coli EC2 ve EC4 suşlarına karşı antimikrobiyal aktivite gösterdiği (sırasıyla P=0,020 ve P=0,026), buna karşın diğer E. coli suşları üzerine zencefil ekstraktlarının herhangi bir etkisi olmadığı (P>0,05) belirlenmiştir. Benzer şekilde, sarımsak etanol ve metanol ekstraktlarının E. coli EC2 suşu dışındaki tüm E. coli suşlarına antibakteriyel etki gösterdiği belirlenmiştir (P<0,05). Bununla birlikte, farklı çözücüler kullanılarak hazırlanan nane ekstraktlarının hiçbir E. coli suşuna karşı antimikrobiyal aktivitesi tespit edilmemiştir. Çalışmanın sonuçları, zencefil etanol veya metanol ekstraktlarının, sarımsak etanol ekstraktlarının çalışılan E. coli suşlarına karşı antimikrobiyal ajan olarak kullanılabileceğini göstermiştir.
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