Biochemical and Histopathological Investigation of the Protective Effect of Misoprostol in Rat Lung Exposed to Paclitaxel
DOI:
https://doi.org/10.24925/turjaf.v11i11.2172-2178.6408Keywords:
Antioxidant, Lung, Misoprostol, Paclitaxel, RatAbstract
In our study, we investigated the protective effect of misoprostol (MP) against paclitaxel (PTX)-induced lung injury in rats. Twenty-one female Sprague-Dawley rats were provided to form 3 groups, each containing seven rats. Control, PTX and PTX + MP. In Control group, was given 1 mL of 0.9% NaCl intraperitoneally (i.p.) and 1 mL of 0.9% NaCl orally for 6 days. PTX and PTX + MP groups were given 2 mg/kg PTX i.p. to each rat on days 0, 2, 4 and 6 of the study. PTX + MP group was given 0.2 mg/kg/day MP orally for 6 days. After tissue samples were taken from rats under anesthesia, biochemical and histopathological analyses were performed on these samples. In the analyses, it was determined that the superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels decreased, while the malondialdehyde (MDA) level increased in the lung tissue of the PTX group rats. In the histopathological examinations, it was determined that histopathological changes such as irregularity and thickening in the alveolar structure, perivascular edema, and inflammatory cell infiltration in the perivascular area occurred in the PTX group rats. In the PTX + MP group, it was observed that the pathological changes detected in the PTX group were mostly prevented. These results show that MP is effective in preventing lung damage caused by PTX, and suggest that using these two drugs together in cancer treatment will be beneficial.
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