Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

Davut Koca; Özlem Orunç Kılınç; Adnan Ayan; Fatma Ertaş Oğuz; Ali Osman Turgut; Özge Oktay Ayan

doi:10.24925/turjaf.v11i12.2389-2392.6447

Authors

Davut Koca Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Van, Türkiye https://orcid.org/0000-0002-7962-6959
Özlem Orunç Kılınç Van Yuzuncu Yil University, Van Yüzüncü Yıl University, Özalp Vocational School of Higher Education, Department of Medical Laboratory Technician, Van, Türkiye Van, Türkiye https://orcid.org/0000-0001-6233-7109
Adnan Ayan Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Genetics, Van, Türkiye https://orcid.org/0000-0002-6564-3416
Fatma Ertaş Oğuz Iğdır University Tuzluca Vocational School, Department of Medical Services and Techniques https://orcid.org/0000-0001-5289-071X
Ali Osman Turgut Siirt University, Faculty of Veterinary Medicine, Department of Animal Science, Siirt, Türkiye https://orcid.org/0000-0001-6863-0939
Özge Oktay Ayan Van Yuzuncu Yil University, Faculty of Medicine, Department of Parasitology, Van, Türkiye https://orcid.org/0000-0003-2577-3774

DOI:

https://doi.org/10.24925/turjaf.v11i12.2389-2392.6447

Keywords:

Abortion, PCR, Sheep, Toxoplasma gondii, Türkiye

Abstract

Toxoplasma gondii, an obligatory intracellular protozoan parasite, can infect a wide range of warm-blooded animals, including livestock species. T. gondii is a zoonotic protozoan parasite that affects both humans and other warm-blooded animals. The aim of this study was to detect T. gondii by using PCR in the brain tissues of 60 aborted sheep fetuses from the Iğdır Province in Türkiye. For this purpose, 60 brain tissue samples of sheep were collected within the lambing seasons of 2023 in Iğdır, Türkiye. The DNA extraction was performed using the PureLink™ Genomic DNA Mini Kit from brain samples. The PCR was performed with the appropriate primers from the obtained DNA samples. T. gondii was found in the brain (16.6%) samples of aborted sheep fetuses. According to the present study, T. gondii infection can be one of the causes of fetus abortion of sheep in Iğdır province, Türkiye. This result emphasizes the need for vigilance and preventive measures in managing this potential public and animal health concerns.

References

Acici M, Babur C, Kilic S, Hokelek M, Kurt M. 2008. Prevalence of antibodies to Toxoplasma gondii infection in humans and domestic animals in Samsun province, Turkey. Tropical Animal Health and Production, 40: 311-315.

Akpinar R, Kiziltepe Ş, Kaya S, Aydin C, Türlek ŞÖ. 2023. Koyun ve Keçi Atık Fetüslerinde Toxoplasma gondii'nin Prevelansının PZR ile Taranması. Firat Universitesi Saglik Bilimleri Veteriner Dergisi, 37(1): 43-48.

Bilgili A, Hanedan B. 2019. Importance of toxoplasmosis for human and animal health, present condition, problems and solution proposals in Turkey and the World. World Journal of Advanced Research and Reviews, 4(2): 61-74.

Anastasia D, Elias P, Nikolaos P, Charilaos K, Nektarios G. 2013. Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. Veterinary Parasitology, 198: 387–390.

Behrem S, Gül S. 2022. Effects of age and body region on wool characteristics of Merino sheep crossbreds in Turkey. Turkish Journal of Veterinary & Animal Sciences, 46(2): 235-247.

Çakmak DÖ, Karatepe B. 2017. Seroprevalence of Toxoplasma gondii in sheep from Nevşehir province in Turkey. Türkiye Parazitoloji Dergisi, 41(3): 148.

Chessa G, Chisu V, Porcu R, Masala G. 2014. Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy. Parasite, 21: 6.

de Moraes ÉBX, da Costa MM, Dantas AFM, da Silva JCR, Mota RA. 2011. Toxoplasma gondii diagnosis in ovine-aborted fetuses and stillborns in the state of Pernambuco, Brazil. Veterinary Parasitology,183(1-2): 152–155.

Edwards JF, Dubey JP. 2013. Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T. gondii from an aborted lamb from a chronically infected ewe. Veterinary Parasitology, 192(1-3): 129-136.

Elmore SA, Jones JL, Conrad PA, Patton S, Lindsay DS, Dubey J. 2010. Toxoplasma gondii: epidemiology, feline clinical aspects, and prevention. Trends in Parasitology. 26: 190–196.

Fuente E, Rodriguez M, Domingo C. 1996. Urine sample used for congenital toxoplasmosis diagnosis by PCR. Journal of Clinical Microbiology, 34(10): 2368–2371.

Greg S J, Vitali GS, David JD. 1996. Comparison of cell culture, mouse inoculation and PCR for detection of Toxoplasma gondii: Effects of storage conditions on sensitivity. Journal of Clinical Microbiology, 34: 1572–1575.

Gutiérrez-Expósito D, Tejerina F, Gutiérrez J, Fernández-Escobar M, Ortega-Mora LM, Mantecón AR, Dagleish MP, Pérez V, Benavides J. 2021. Direct economic losses of Toxoplasma gondii abortion outbreaks in two Spanish sheep flocks. Veterinary Parasitology: Regional Studies and Reports, 26: 100623.

Innes E. 2010. A brief history and overview of Toxoplasma gondii. Zoonoses Public Health 57: 1–7.

Irehan B, Sonmez A, Atalay MM, Ekinci AI, Celik F, Durmus N, Turkan Ciftci A, Simsek S. 2022. Investigation of Toxoplasma gondii, Neospora caninum and Tritrichomonas foetus in abortions of cattle, sheep and goats in Turkey: Analysis by real-time PCR, conventional PCR and histopathological methods. Comparative Immunology, Microbiology and Infectious Diseases, 89: 101867.

Kapperud G, Jenum PA, Stray-Pedersen B. 1996. Risk factors for Toxoplasma gondii infection in pregnancy. Results of a prospective case-control study in Norway. American Journal of Epidemiology, 144: 405–412.

Orunç Kılınç Ö, Ayan A, Yumuşak N, Kömüroğlu AU, Aslan B, Çelik ÖY, Göz Y. 2023. Investigation of Toxoplasma gondii and Neospora caninum in different tissues of aborted foetuses of sheep in Van Province, Türkiye: Analysis by nested PCR, histopathological and immunohistochemical methods. Acta Veterinaria Brno, 92(2): 123-131.

Köseman A, Şeker İ. 2015. Current status of cattle, sheep and goat breeding in Turkey. Van Veterinary Journal, 26(2): 111-117.

Moreno B, Collantes-Fernández E, Villa A, Navarro A, Regidor-Cerrillo J, Ortega-Mora L. 2012. Occurrence of Neospora caninum and Toxoplasma gondii infections in ovine and caprine abortions. Veterinary Parasitology. 187(1-2): 312–318.

Nayeri T, Sarvi S, Moosazadeh M, Daryani A. 2021. Global prevalence of Toxoplasma gondii infection in the aborted fetuses and ruminants that had an abortion: A systematic review and meta-analysis. Veterinary parasitology, 290: 109370.

Ndao, M. 2009. Diagnosis of parasitic diseases: old and new approaches. Interdisciplinary perspectives on infectious diseases, 2009.

Oncel T, Vural G. 2006. Occurrence of Toxoplasma gondii antibodies in sheep in Istanbul, Turkey. Veterinarski Arhiv, 76(6): 547-553.

Özkaraca M, İrehan B, Parmaksiz A, İtik Ekinci A, Çomakli S. 2016. Koyun ve Keçi Abortlarında Neospora caninum ve Toxoplasma gondii'nin Dubleks PCR, İmmunohistokimyasal ve İmmunfloresans Yöntemlerle Teşhisi. Ataturk University Journal of Veterinary Sciences, 11(2): 200-206.

Paştiu AI, Mircean V, Mercier A. 2023. Toxoplasma gondii infection in sheep from Romania. Parasites Vectors, 16: 24.

Rassouli M, Razmi GR, Bassami MR, Movassaghi AR, Azizzadeh M. 2011. Study on ovine abortion associated with Toxoplasma gondii in affected herds of Khorasan Razavi Province, Iran based on PCR detection of fetal brains and maternal serology. Parasitology. 138(6): 691-697.

Sah RP, Dey AR, Rahman AA, Alam MZ, Talukder MH 2019: Molecular detection of Toxoplasma gondii from aborted fetuses of sheep, goats and cattle in Bangladesh. Veterinary Parasitology: Regional Studies and Reports, 18: 100347

Salehi M, Nezami H, Niazkar HR. 2020. Investigation of Toxoplasma gondii infection in aborted fetuses of sheep using PCR: A study in North Khorasan Province, Iran. Veterinary Medicine International, 2020: 1-5.

Shahbazi G, Rad NH, Madani R, Matin S, Mortazavi P, Jangjou AH. 2019. Toxoplasma gondii in aborted fetuses of sheep in Ardebil Area, North-West of Iran. Iranian Journal of Parasitology, 14(3): 430.

Steuber S, Niu A, Bauer C, Reetz J, Roth A, Janitschke K. 1995. The detection of Toxoplasma gondii in abortion tissues of sheep using the polymerase chain reaction. Deutsche Tierarztliche Wochenschrift, 102(2): 91–93.

Tavassoli M, Esmaeilnejad B, Tabatabaei M. 2009. A survey on infection of animals with Toxoplasma gondii using PCR and genetic differences using RFLP in Urmia, Iran. Pazhoohesh va Sazandegi, 58: 61–66.

Tenter AM, Heckeroth AR, Weiss LM. 2000. Toxoplasma gondii: from animals to humans. International Journal for Parasitology, 30: 1217-1258.

TUIK (2022). Hayvansal Üretim İstatistikleri. Link: https://data.tuik.gov.tr/, accession date: 16.10.2023.

Tutuncu M, Ayaz E, Yaman M, Akkan HA. 2003. The seroprevalance of Toxoplasma gondii in sheep, goats and cattle detected by indirect haemaglutinnation (IHA) test in the region of Van, Turkey. Indian Veterinary Journal, 80: 401-403.

Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye

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