The Effect of Olive Oil Industry By-Product Wastewater on the Quality and Microbiological Characteristics of Hungarian-triticale Silages

Authors

DOI:

https://doi.org/10.24925/turjaf.v12i7.1178-1184.6679

Keywords:

Hungarian vetch, olive wastewater, Silage, fermentation, microbiology

Abstract

The aim of this study was to investigate the effect of the addition of olive wastewater, a by-product of olive oil industry, to Hungarian vetch-triticale silages at different doses on their chemical composition, fermentation, physical, microbiological properties and in vitro digestibility. The experiment silages were prepared as follows: (i) 100%Hungarian vetch-triticale silage (control); (ii) 95%Hungarian vetch-triticale + 5%olive wastewater; (iii) 90%Hungarian vetch-triticale + 10%olive wastewater; (iv) 85%Hungarian vetch-triticale + 15%olive wastewater; (v) 80%Hungarian vetch-triticale + 20%olive wastewater. The ensiling period continued for 56 days. As a result, odor, organic matter, pH and water-soluble carbohydrate contents of silages decreased; dry matter, crude ash, ADF, ether extract and Fleig Score increased by olive wastewater addition. Differences in crude protein, crude fiber, NDF, ammonia nitrogen, lactic acid bacteria, in vitro dry matter and organic matter digestibility were not significant. Sulfite reducing anaerobes, Listeria spp., Enterobactericeae and yeasts were not found in the experiment silages. Mold content was found in one sample in the group containing 15%olive wastewater. At the end of the study, it was concluded that the addition of up to 20%olive wastewater to Hungarian vetch-triticale mixtures did not have any negative effect.

References

Aksu Elmalı, D. & Duru M. (2012). Effects of different levels of malic acid on the some quality and micro flora of vetch (Vicia sativa L.) Silage. Journal of Animal and Veterinary Advances, 11 (10): 1651-1655.

Alburquerque, J.A., Gonzalez, J., Garcia, D. & Cegarra, J. (2004). Agrochemical characterisation of “alperujo”, a solid by-product of the two-phase centrifugation method for olive oil extraction. Bioresource Technology. 91 (2): 195–200.

Anonim, 2024. Zeytin. https://biruni.tuik.gov.tr/medas/?locale=tr (Erişim tarihi: 26.02.2024)

AOAC (1999). Official Methods of analysis, 16th ed., 4th revision, Association of Official Analytical Chemists, Washington, DC, USA.

Arican, G. & Arslan Duru, A. (2020). The effect of black seed oil (Nigella sativa) on chemical, fermentation and in vitro digestibility of hungarian vetch-wheat silage. Pakistan Journal of Botany. 52(2): 723-727.

Arslan M., Erdurmuş, C., Öten, M., Aydınoğlu, B. & Çakmakçı S. (2016). Mısır ile soyanın farklı oranlarda karıştırılmasıyla elde edilen silajlarda besin değerinin belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 31 (3): 417-422.

Arslan Duru, A., Aksu Elmalı, D., Yüksel, O. & Kutay, H. (2021). The effects of addition malic acid and whey on some parameters of vetch-wheat silages. Fresenius Environmental Bulletin 30 (09):10435-10440.

Bingol, N.T., Karsli, M.A., Bolat, D. & Akca, I. (2008) The effects of molasses and formic acid addition in sainfoin silage harvested at different maturities on silage quality and digestibility. Van Veterinary Journal. 19(2): 61-66.

Broderick, G.A. & Kang, J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminalfluid and in vitro media. J. Dairy Sci., 63: 64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8

Bufano, G., Cianci, D., Montemurro, O., Palermo, D. & Tasca, M.L. (1982). Prove di razionamento degli ovini con paste di vegetazione dei frantoi oleari. Scienza e tecnica Agraria, 1-2: 1-10.

Demirci, U., Gülşen, N. & Keleş, G. (2011). Effects of bacterial inoculants on fermentation and aerobic stability of baled triticale-hungarian vetch silage and lamb performance. Kafkas Univ. Vet. Fak. Derg., 17(2): 297-302.

Demirel, M., Cengiz, F., Çelik, S. & Erdoğan, S. (2001). Van ekolojik koşullarında yetiştirilen mısır ve macar fiği karışımlarının silaj kaliteleri ve besin maddelerinin rumende parçalanabilirlikleri üzerine bir araştırma, Yüzüncü Yıl Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisi, 11 (1): 69-78.

DLG. (1987). Energie- und Na¨hrstoffbedarf landwirtschaftlicher Nutztiere. 4. Schweine. DLG-Verlag GmbH, Frankfurt, Germany.

D’Orleans, M., Giger, S. & Sauvant, D. (1980). Mise au point d’une methode enzmatique de prevision de la digestibilite de la matiere organique des aliments concentres. Institut National Agronomique. Paris. Grignon. 1980.

Dubois, M., Gilles, K.A., Hamilton, J.K., Rebbers, P.A. & Smith. F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28: 350-356.

Dumlu, Z. & Tan, M. (2009). Erzurum şartlarında yetişen bazı baklagil yem bitkileri ve karışımlarının silaj değerlerinin belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 40 (2): 15-21.

Durgut, G. (2008). Etlik piliç yemlerine karıştırılan zeytin karasuyunun piliçlerin bazı verim özellikleri ile et kalite özelliklerine etkisi. Adnan Menderes Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 57 s. Aydın.

Ellis, J. L., Bannink, A., Hindrichsen, I. K., Kinley, R. D., Pellikaan, W. F., Milora, N. & Dijkstra, J. (2016). The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Animal Feed Science and Technology, 211: 61–74. https://doi.org/10.1016/j.anifeedsci.2015.10.016

Ergün A., Tuncer Ş. D., Çolpan İ., Yalçın S., Yıldız G., Küçükersan M. K., Küçükersan S., Şehu A. & Saçaklı P. (2013). Yemler Yem Hijyeni ve Teknolojisi. Pozitif matbaacılık, Ankara, Türkiye.

Filya, İ. & Sucu, E. (2005). Silaj fermantasyonunda organik asit kullanımı üzerinde araştırmalar 1. Formik asit temeline dayalı bir koruyucunun laboratuvar koşullarında yapılan mısır silajlarının fermantasyon, mikrobiyal flora, aerobik stabilite ve in situ rumen parçalanabilirlik özellikleri üzerine etkisi, Tarım Bilimleri Dergisi, 11 (1): 51-56. DOI: 10.1501/Tarimbil_0000000487

Gümüşkesen, A.S. (1999). Bitkisel Yağ Teknolojisi. Asya Tıp Yayıncılık Ltd., 182s, İzmir.

Harrigan, W.F. (1998). Laboratory Methods in Food Microbiology, Third Ed., Academic Pres. London.

İkizoğlu, E. (2009). Zeytinyağı işletmesi atık suyu (karasu) bertarafı, artırılması ve değerlendirilmesi. Zeytinyağı. Eflatun Yayınevi. 139-155, Ankara.

Kanengoni, A.T., Nkosi1, B.D., Chimonyo, M., Ndimba, B. & Dzama, K. (2016). Effects of whey, molasses and exogenous enzymes on the ensiling characteristics, nutrient composition and aerobic stability of maize cobs. South African Journal of Animal Science, 46 (2): 113-120. https://doi.org/10.4314/sajas.v46i2.1

Karakozak, E. & Ayaşan, T. (2010). Değişik yem bitkileri ve karışımlarından hazırlanan silajlarda inokulant kullanımının flieg puanı ve ham besin maddeleri üzerine etkileri. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 16 (6): 987-994. DOI: 10.9775/kvfd.2010.2197

Kılıç, A. (1986). Silo yemi (öğretim, öğrenim ve uygulama önerileri). Bilgehan Basımevi, İzmir, 357.

Kılıçalp, N., Özkurt, M., Karadağ, Y., & Hızlı, H. (2022). Hungarian vetch (Vicia pannonica) and triticale (Xtriticosecale wittmack) silages, sowed in different seed rates, treated with lactic acid bacteria+ enzyme mixture inoculant: I. Chemical composition and silage Fleig score. Journal of Animal & Plant Sciences, 32(3): 680-687.

Koç, F., Coşkuntuna, L., Özdüven M.L. & Coşkuntuna, A. (2010). Farklı ortam sıcaklıklarında organik asit kullanımının fiğ-tahıl silajlarında fermantasyon gelişimi ve aerobik stabilite üzerine etkileri, Tekirdağ Ziraat Fakültesi Dergisi, 7(2): 159-165.

Martillotti, F. (1983). Use of olive by –products in animal feeding in Italy. Int. Seminar on olive oil technology and valorization of by – products. 20 – 24 October 1986. Izmir – Turkey.

McDonald, P., Henderson, A. R. & Herson, S. J. E. (1991). The Biochemistry of Silage. 16th ed.,Chalcombe Publication, Marlow, UK.

Menke, K.H. & Steingass, H. (1998). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev. 28: 7-55.

Molina-Alcaide, E. & Yanez-Ruiz, D. (2008). Potential use of olive by-products in ruminant feeding: A Review. Animal Feed Science and Technology. 147 (1): 247-264. https://doi.org/10.1016/j.anifeedsci.2007.09.021

Nkosi, B.D., Groenewald, I.B., Meeske, R., Motiang, M.D. & Langa, T. (2013). Effects of inoculating citrus (Citrus sinensis) tree leaves with either bacterial or whey on the fermentation and aerobic stability of silage. African Journal of Agricultural, 8(9): 792-796. https://doi.org/10.5897/AJAR12.999

Polan, C.E., Stive, D.E. & Garrett, J.L. (1998). Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant, Journal of Dairy Science, 81: 765-776. https://doi.org/10.3168/jds.S0022-0302(98)75633-4

Sansoucy, R. (1985). Olive by-products for animal feed. FAO: Food and Agriculture Organization, Animal Production and Health, Paper 43.pp Rome.

Şahin K., Çerçi, İ.H., Güler, T., Şahin, N., Kalander, H. & Çelik, S. (1999). Farklı silaj katkı maddelerinin yas şeker pancarı posası silajı kalitesine etkileri, Tr. J. of Veterinary and Animal Sicences, 23: 285-292.

Verna, M., Martillotti, F. & Puppo, S. (1988). Compozisione e valore nutritivo del residuo concentrato dele acque di vegetazione dei frantoi oleari. Ann. Ist. Sper. Zoot., 21: 147-156.

Van Soest, P. J. (1982). Analytical systems for evaluation of feeds. In: P. J. Van Soest (Editor), Nutritional Ecology of the Ruminant. Cornell University Press. Chapter 6: 75-94. Ithaca, NY.

Vlyssides, A.G., Loizides, M. & Karlis, P.K. (2004). Integrated strategic approach for reusing olive oil extraction by-products. Journal of Cleaner Production. 12: 603–611. https://doi.org/10.1016/S0959-6526(03)00078-7

Yeşilada. Ö., Şık, S. & Sam, M. (1998). Biodegradation of olive oil mill wastewater by coriolus versicolor and funalia trogii: Effects of Agitation. initial cod concentration, inoculum size and immbolization, Worl J. Microbiol.Biotechnol., 14: 37-42. https://doi.org/10.1023/A:1008816231563

Published

14.07.2024

How to Cite

Arslan Duru, A., Aksu Elmalı, D., Yüksel, O., & Duru, M. (2024). The Effect of Olive Oil Industry By-Product Wastewater on the Quality and Microbiological Characteristics of Hungarian-triticale Silages. Turkish Journal of Agriculture - Food Science and Technology, 12(7), 1178–1184. https://doi.org/10.24925/turjaf.v12i7.1178-1184.6679

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Research Paper

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