Detection of Prunus Necrotic Ringspot Virus (PNRSV) and Apple Mosaic Virus (ApMV) in Rose (Rosa spp.) Plants in Konya Province
DOI:
https://doi.org/10.24925/turjaf.v13i1.71-77.7335Keywords:
ApMV, DAS-ELISA, Konya, Mosaic, PNRSV, RoseAbstract
This research was conducted to determine the presence of Prunus necrotic ringspot virus (PNRSV) and Apple mosaic virus (ApMV) that can cause infection in rose (Rosa spp.) plants grown in Konya province. For this purpose, field and laboratory studies were carried out in areas where roses are mostly grown for landscaping purposes, and infection rates were calculated with the data obtained. The hypothesis of the study is that roses in Konya province may be infected with PNRSV and ApMV and the presence of these viruses can be determined. In line with this hypothesis, various rose growing areas in Konya province were selected as the research area. During the field studies in 2023, guided sampling was carried out and 94 leaf, branch and flower samples were collected from different rose varieties. The collected samples were tested for determining of PNRSV and ApMV infections by Double Antibody Sandwich (DAS) ELISA method, which is one of the serological testing methods in the laboratory. As a result of the tests, it was revealed that the single infections of PNRSV and ApMV were present in 12 and 11 samples of rose plants in Konya province. The total infection rate of both viruses in the province was calculated as 24.47%. Also, PNRSV+ApMV mixed infections were detected in 2 samples. In this study, the infections of PNRSV and ApMV on roses in Konya province were determined for the first time by serological methods. These results will serve as an important source of information for rose producers and agricultural engineers in the region and will allow the development of strategies to control the spread of viruses and minimize infections.
References
Akbaş, B., & Ilhan, D. (2005). Türkiye’de elmada elma mozaik virüsünün yaygın dağılımı. Bitki hastalığı, 89 (9), 1010-1010.
Akgül, S., Gazel, M., Tunç, B., & Çağlayan, K. (2021). Adıyaman ili badem ağaçlarını enfekte eden önemli Prunus virüslerinin DAS-ELISA ve RT-PCR analizleri ile saptanması ve karakterizasyonu. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 26(3), 576-585.
Akpınar, G. (2009). Türkiye’de Trakya bölgesinde güllerde görülen virüslerin biyolojik ve serolojik yöntemlerle saptanması üzerine araştırmalar (Yüksek Lisans tezi, Namık Kemal Üniversitesi).
Amet, M. C. (2021). Tekirdağ ilinde üretimi yapılan sert çekirdekli meyve ağaçlarında Plum pox virus (PPV) ve Prunus necrotic ringspot virus (PNRSV)’lerinin saptanması üzerine araştırmalar (Yüksek Lisans Tezi, Tekirdağ Namık Kemal Üniversitesi).
Anonim, (2022). Tarım Ürünleri Piyasaları-Gül, Tarımsal Ekonomi ve Politika Geliştirme Enstitüsü (TEPGE). https://arastirma.tarimorman.gov.tr/tepge/Belgeler/PDF%20Tar%C4%B1m%20%C3%9Cr%C3%BCnleri%20Piyasalar%C4%B1/2022-ocak%20Tar%C4%B1m%20%C3%9Cr%C3%BCnleri%20Rapor%C4%B1/G%C3%BCl,%20Ocak-2022%20Tar%C4%B1m%20%C3%9Cr%C3%BCnleri%20Piyasa%20Raporu--+.pdf
Anonim, (2024). Türkiye İstatistik Kurumu. Bitkisel Üretim İstatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr
Arli Sökmen, M., Kutluk Yimaz N.D., Mennan H., & Sevik M.A. (2005). Natural Weed Hosts of Apple mosiac virus in Hazelnut Orchards in Turkey. Journal of. Plant Pathology, 87 (3), 239-242.
Bjarnason, E. N., Hanger, B. C., Moran, J. R., & Cooper, J. A. (1985). Production of prunus necrotic ringspot virus-Free Roses by Heat Treatment and Tissue Culture. New Zealand Journal of Agricultural Research, 28: 151- 156
Canik, D., Ertunç, F., Gospodaryk, A., Budzanivska, İ., & Polischuk, V. P. (2011). Elma Mozaik Ilarvirusu Türkiye ve Ukrayna İzolatlarının Moleküler Karakterizasyonu. Journal of Agricultural Sciences, 17(2). https://doi.org/10.1501/Tarimbil_0000001161
Clark, M.F., & Adams, A.N. (1977). Characteristic of Microplate Method of Enzyme Linked Immunosorbent Assay for the Detection of Plant Viruses, Journal of Gen. Virol., 34: 475-483.
Çağlayan K, Ulubaş Serçe Ç, Gazel M, & Jelkmann W. (2006). Detection of four Apple Viruses by ELISA and RT-PCR Assays in Turkey. Turkish. J. of Ag. For. 30:241-246.
Çelik, A., & Ertunç, F. (2019). First report of Prunus necrotic ringspot virus infecting apple in Turkey. Journal of Plant Pathology, 101(4), 1227-1227.
Erdiller, G., Elibüyük, İ. Ö., & Akbaş, B. (1995). Güllerde Görülen Virüs Hastalıkları. Türkiye Fitopatoloji Kongresi Bildirileri, 26-29 Eylül Adana s. 286-289.
Fulton R.W. (1981)- Ilarviruses. - In Handbook of plant virus infections and comparative diagnosis. (E. Kurstak. ed.)
Fulton, R.W. (1970). Prunus Necrotic Ringspot Virus. CMI/ABB Description of Plant Viruses. No. 5.
Güran, S. (2007). Şanlıurfa ilinde güllerde (Rosa spp.) erik nekrotik halkalı leke virüsü (PNRSV)’nün ELISA ve RT-PCR yöntemleriyle saptanması (Yüksek lisans tezi, Harran Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı).
Kısa, N., & Korkmaz, S. (2021). Çanakkale İlinde Rose Yellow Vein Virus Etmeninin Tespiti ve Genetik Çeşitliliği. Anadolu Tarım Bilimleri Dergisi, 36(3), 446-453.
Korkmaz, M., Özçelik, H., Kandemir, A., & İlhan, V. (2013). Erzincan ve çevresinde yayılış gösteren doğal Gül (Rosa L.) taksonları. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17(1), 49-59.
Manners, M.M. (1997). Effects of rose mosaic disease on performance of hybrid tea roses in Florida. Proc. Fla. State Hort. Soci. 110: 118-121.
Milleza, E. J. M., Ward, L. I., Delmiglio, C., Tang, J. Z., Veerakone, S., & Perez-Egusquiza, Z. (2013). A survey of viruses infecting Rosa spp. in New Zealand. Australasian Plant Pathology, 42, 313-320.
Moury, B., Cardın, L., Onesto, J.-P., Candresse, T., & Poupet, A. (2001). Survey of prunus necrotic ring spot virus in Rose and its variability in rose and Prunus spp. Phytopathology, 91:84-91.
Nemeth, M. (1986). Virus, Mycroplasma and Ricettsia Diseases of Fruit Trees. Martinus Nijhoff Publishers. The Netherlands and Academi Kiado, Hungary. Dr. W. Junk Publishes p.841.
Nyland, G., Gilmer, R. M. & Moore, J. D. (1976). Prunus Ringspot Group. Pages: 104-132. In: Virus Diseases and Noninfectious Disorders of Stone Fruits in North America. U.S. Dep. Agric. Handbook: 437.
Öztekin, V., & Buzkan, N. (2012). Prevalance of Stone Fruit Viruses in Almond Orchard of Kahramanmaras Sütçü Imam University. KSÜ Doğa Bilimleri Dergisi, 15(1), 30-33.
Paz, M. L., Sivaprasad, Y., Garrido, P., Ayala, L., Méndez, K., Garrido, A., & Flores, F. (2020). First report of apple mosaic virus infecting Rosa spp. in Pichincha province, Ecuador. Journal of Plant Pathology, 102, 1359-1359.
Shiel P.J. & Berger, P.H. (2000). The Complete Nucleotide Sequence of Apple Mosaic Virus (ApMV) RNA 1 and RNA 2: ApMV is More Closely Related to Alfalfa Mosaic Virus Than to Other Ilarviruses. Journal General Virology, 81: 273-278.
Sipahioglu, H.M., B.K. Cağlar & Baloğlu, S. (2001). Doğu Akdeniz Bölgesi’nde Güllerde Zararlı PNRSV ve ApMV Virüs Hastalıklarının Serolojik Olarak Yaygınlıklarının Saptanması. Türkiye IX Fitopatoloji Kongresi Bildiriler Kitabı, 3-8 Eylül 2001, Tekirdağ, Trakya Üniversitesi Yayınları No:45: 572-577
Szyndel, M. S., Paduch-Cıchal, E., & Sala-Rejczak, K. (2006). Viruses causing diseases on roses. Ochrona Roslin, 51(2): 34-37.
Şimşek, G., Akçağlar, S., & Shenavai, S. (2010). Trizol RNA Ekstraksiyon Metodu: İnek plasentomu için oldukça etkili metod değerlendirmesi. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 29(1), 1-6.
Tan, J. L., Trandem, N., Fránová, J., Hamborg, Z., Blystad, D. R., & Zemek, R. (2022). Known and potential invertebrate vectors of raspberry viruses. Viruses, 14(3), 571.
Thomas, B. J. (1980). The Detection by Serological Methods of Viruses Infecting The Rose. Ann. App. Biol., 94: 91-101.
Torusdağ, G.B. & Bakkalbaşı, E., 2019, Gül (Rosa spp.) Bitkisine Genel Bir Bakış, International Conference on Agriculture, Animal Scıence and Rural Development-III, Van, 771-783.
Uzunoğulları, N., & İlbağı, H. (2009). Güneydoğu Marmara Bölgesinde Yumuşak Çekirdekli Meyvelerde Apple mosaic ilarvirus (ApMV)’un Saptanması. Bahçe, 38(1), 9-14.
Wani, LA., Jawa, P. & Khan, JA. (2022). Hindistan’dan Gül türlerinin Elma mozaik virüsü ve Prunus nekrotik halkalı leke virüsü enfeksiyonu açısından taranması ve bunların eş zamanlı tespiti için bir dupleks RT-PCR testinin tasarlanması. Int. J. Biotechnol. Microbiol., 4 (1), 77-86.
Yardımcı, N., & Çulal-Kılıç, H. (2009). Tomato spotted wilt virus in vegetable growing areas in the west mediterranean region of Turkey. African Journal of Biotechnology, 8(18), 4539-4541.
Yardımcı, N., & Kılıç, H. Ç. (2010). Isparta İlinde Yağlık Güllerde (Rosa damascena) Strawberry latent ringspot virus. Ziraat Fakültesi Dergisi, 5(2), 22-26.
Yardımcı, N., & Eryiğit, H. (2006). Isparta İli Elma Üretim Alanlarında Apple Mosaic Virus (Elma Mozayik Virüsü) (ApMV)’nün Belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2), 185-187.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
