Yaygın Olarak Tüketilen Helianthus tuberosus, Zingiber officinale ve Raphanus sativus var. niger Bitkilerinde in Vitro Antioksidan Enzim Kapasitesi ve Oksidatif Stres Düzeylerinin Belirlenmesi
DOI:
https://doi.org/10.24925/turjaf.v11i11.2110-2115.6272Anahtar Kelimeler:
Antioksidan aktivite MDA Oksidatif stres Bitkisel terapötikler Zingiber officinale - Antioksidan aktivite - MDA - Oksidatif stres - Bitkisel terapötikler - Zingiber officinaleÖzet
Günümüzde kullanılan birçok terapötik ilaç kaynağını şifalı bitkilere borçludur. Her bitkinin kendine özgü aktivitesi ve biyokimyasal profili vardır. Bu çalışmada günlük hayatta oldukça iyi bilinen üç farklı bitki türü; Helianthus tuberosus, Zingiber officinale ve Raphanus sativus var. niger kullanıldı. İlk defa yapılan bu çalışmada antioksidan aktivite tayini sınırlı olan üç farklı bitki seçilmiş ve aktioksidan kapasiteleri karşılaştırılmıştır. H. tuberosus, Z. officinale ve R. sativus var. niger bitkilerinin gövde kısımları %1.15 potasyum klor ile homojenize edilerek ekstraklar hazırlandı. Daha sonra antioksidan enzim aktiviteleri katalaz (CAT) ve süperoksit dismutaz (SOD) ile oksidatif stres belirteci olan malondialdehit (MDA) düzeyleri spektrofotometrik yöntemlerle saptandı. Araştırma sonucunda en yüksek SOD enzim aktivitesi R. sativus var. niger bitkisinde görülürken, en yüksek CAT enzim aktivitesi ise Z. officinale bitkisinde gözlendi. Öte yandan yer elması bitki ekstraktında MDA düzeyi en yüksek iken, en düşük MDA düzeyi ise Z. officinale bitkisinde gözlenmiştir.
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