Determination of Bacteria Levels Contained in Some Probiotic Preparations Consumed and Confirmation by PCR
DOI:
https://doi.org/10.24925/turjaf.v12i1.59-65.6400Keywords:
Bifidobacterium spp. , Lactobacillus spp. , Probiotic microorganisms , Probiotic-prebiotic preparation , PCRAbstract
This study aimed to investigate the viability and levels of bacteria as indicated on the label in probiotic preparations used as supplements. Forthis purpose, a total of 15 different preparations containing LactobacillusandBifidobacterium species belonging to different brands were obtained. The sampleswere subjected to microbiological cultivation in terms of Lactobacillus andBifidobacterium species. As a result of the microbiological analysis, live bacteria were detected in 13 (%87) of the samples, while no growth was observed in 2 (%13) samples. Lactobacillus spp. average number of bacteria in probiotic preparations containing 5,9×1010 cfu/g;Bifidobacterium spp.on the other hand, in probiotic preparations containing bacteria, the average number of bacteria was 1,3×109 cfu/g. Strains that underwent Gram staining, catalase test and carbonhydrate fermentation test were confirmed by PCR analysis. As a result of PCR analysis, Lactobacillus strains and Bifidobacterium strains were confirmed in 13 samples. As a result, the presence, viability, and quantity of strains specified in ready-to-use probiotic preparations are very important. Our study showed that the accuracy of the information written on the label was 87%.
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