Tüketilen Bazı Probiyotik Preparatların İçerdiği Bakteri Seviyelerinin Belirlenerek PZR ile Doğrulanması
DOI:
https://doi.org/10.24925/turjaf.v12i1.59-65.6400Anahtar Kelimeler:
Bifidobacterium spp. - Lactobacillus spp. - Probiyotik mikroorganizmalar - Probiyotik-prebiyotik preparatlar - PZRÖzet
Bu çalışma, takviye olarak kullanılan probiyotik preparatların etikette belirtildiği gibi içerdiği bakterilerin canlılık ve seviyelerinin incelenmesi amacıyla yapılmıştır. Bu amaçla farklımarkalara ait Lactobacillus ve Bifidobacterium türlerini içeren toplam 15 farklı preparat temin edildi. Örnekler Lactobacillus ve Bifidobacterium türlerini yönünden mikrobiyolojik ekime tabii tutuldu. Yapılan mikrobiyolojik analiz sonucunda örneklerin 13’ünde (%87) canlı bakteri saptanırken 2 örnekte (%13) üreme olmadığı gözlemlendi. Lactobacillus spp. içeren probiyotik preparatlarda bakteri sayısı ortalama 5,9×1010 kob/g; Bifidobacterium spp. içeren probiyotik preparatlarda ise bakteri sayısının ortalama 1,3×109 kob/g olduğu görüldü. Gram boyama, katalaz testivekarbonhidrat fermentasyon testi yapılan suşların Polimeraz Zincir Reaksiyonu (PZR) analiziile doğrulamaları yapıldı. PZR analizi sonucunda, 13 örnekte Lactobacillus ve Bifidobacterium türleri tespit edildi. Sonuç olarak tüketime sunulan hazır probiyotik preparatlarda belirtilen suşların varlığı, canlılığı ve miktarı oldukça önemlidir. Etikette yazılan bilgilerin doğruluk payının %87 olduğu yaptığımız çalışmamızda görüldü.
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